Detailed information NTG0233564

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: CDO11_RS08435
• Genome accession: NZ_CP021789
• Coordinates: 1657867..1659600 (+)
• Length: 577 a.a.
• NCBI ID: WP_011408072.1
• Uniprot ID: A0A0K0GIN1
• Organism: Xanthomonas oryzae pv. oryzae strain PXO61
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1643206..1657832	1657867..1659600	flank	35

Gene organization within MGE regions

Location: 1643206..1659600

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CDO11_RS24875	-	1643206..1643535 (+)	330	WP_125168748.1	hypothetical protein	-
CDO11_RS08350 (CDO11_08175)	-	1643612..1645045 (+)	1434	Protein_1513	RHS repeat-associated core domain-containing protein	-
CDO11_RS08355 (CDO11_08180)	-	1645051..1645419 (+)	369	WP_011408064.1	DUF6869 domain-containing protein	-
CDO11_RS08360 (CDO11_08185)	-	1645589..1646557 (-)	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
CDO11_RS08365	-	1646653..1646820 (+)	168	Protein_1516	IS701 family transposase	-
CDO11_RS08375 (CDO11_08195)	-	1646938..1647159 (+)	222	Protein_1517	IS4 family transposase	-
CDO11_RS08380 (CDO11_08200)	-	1647249..1648205 (+)	957	WP_041182041.1	IS30-like element IS1112a family transposase	-
CDO11_RS08385 (CDO11_08205)	-	1648189..1648491 (+)	303	WP_158645227.1	hypothetical protein	-
CDO11_RS08395 (CDO11_08210)	-	1649052..1650371 (-)	1320	WP_109181905.1	IS701-like element ISXo15 family transposase	-
CDO11_RS08400 (CDO11_08215)	coaE	1650442..1651050 (-)	609	WP_011258357.1	dephospho-CoA kinase	-
CDO11_RS08405 (CDO11_08220)	-	1651064..1651927 (-)	864	WP_011258358.1	A24 family peptidase	-
CDO11_RS08410 (CDO11_08225)	pilC	1651934..1653193 (-)	1260	WP_011408067.1	type II secretion system F family protein	Machinery gene
CDO11_RS08415 (CDO11_08230)	pilA2	1653548..1653973 (+)	426	WP_011408068.1	pilin	Machinery gene
CDO11_RS08420 (CDO11_08235)	-	1654108..1655697 (+)	1590	WP_011408069.1	phosphoethanolamine transferase	-
CDO11_RS08425 (CDO11_08240)	-	1655708..1656337 (+)	630	WP_011408070.1	hypothetical protein	-
CDO11_RS26070 (CDO11_08245)	-	1656307..1657832 (+)	1526	Protein_1527	hypothetical protein	-
CDO11_RS08435 (CDO11_08250)	pilB	1657867..1659600 (+)	1734	WP_011408072.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 577 a.a.
• Molecular weight: 62970.00 Da
• Isoelectric Point: 5.4513

>NTDB_id=233564 CDO11_RS08435 WP_011408072.1 1657867..1659600(+) (pilB) [Xanthomonas oryzae pv. oryzae strain PXO61]
MNSVSTTNLVGITGIARRLVQDGALEETAARTAMEQAAAAKVPLPQWFAENRLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD

Nucleotide

• Length: 1734 bp

>NTDB_id=233564 CDO11_RS08435 WP_011408072.1 1657867..1659600(+) (pilB) [Xanthomonas oryzae pv. oryzae strain PXO61]
ATGAATTCAGTATCTACAACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AACCGCTGCGCGGACAGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAACAGGCTGG
TGTCGGCGTCGCAGCTTGCGGCGGCCAATGCAGTGGAATTTGGCATGCCACTGATGGATGTGTCTGTGTTCGACGCTAAC
CAGAACGCAGTCAAGCTAGTCAGCGAGGAGTTGCTCCAGAAGTACCATGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACTCAGACCCGTGCACTAGATGACATCAAGTTTCATACGAATCTGGTCGTCGAGCCGA
TTCTTGTCGATGAGGACCAAATCCGTCGCACATTGGAGCAGTGGCAGGCAAGCAATGCCTCATTTGGATCATCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCCACCGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGGGCCTCGGACA
TCCATTTCGAGCCGTACGAAGACGACTACCGGGTGCGGTTGCGGATCGACGGGTTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCT
GTACACGGCGCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATTCGCCTGCCTG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTTGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGGATCGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAATAACTGCAAGCGCAAGTCGACGTTGCCG
GAGCATGCACTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTCGCGAATATGGAGCTTTATGAAGCCGTGGGTTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCCGCTCAGAAGATCGGCATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCAAGGCGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-152)

3D structure

• AlphaFold DB: A0A0K0GIN1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	57.042	98.44	0.562
pilB	Acinetobacter baumannii D1279779	56.69	98.44	0.558
pilB	Legionella pneumophila strain ERS1305867	52.465	98.44	0.516
pilB	Vibrio cholerae strain A1552	49.74	100	0.497
pilF	Neisseria gonorrhoeae MS11	49.648	98.44	0.489
pilB	Vibrio campbellii strain DS40M4	46.046	98.614	0.454
pilB	Vibrio parahaemolyticus RIMD 2210633	46.359	97.574	0.452
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.394	91.508	0.36