Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CSB90_RS03570 Genome accession   NZ_CP027172
Coordinates   754116..754739 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain AR_0353     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 749116..759739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSB90_RS03555 (CSB90_0719) - 749803..752274 (-) 2472 WP_003135771.1 penicillin-binding protein 1A -
  CSB90_RS03560 (CSB90_0720) pilM 752459..753523 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  CSB90_RS03565 (CSB90_0721) pilN 753523..754119 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  CSB90_RS03570 (CSB90_0722) pilO 754116..754739 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  CSB90_RS03575 (CSB90_0723) comL/pilP 754736..755260 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  CSB90_RS03580 (CSB90_0724) pilQ 755314..757458 (+) 2145 WP_003135767.1 type 4a pilus secretin PilQ Machinery gene
  CSB90_RS03585 (CSB90_0725) aroK 757470..757988 (+) 519 WP_003095833.1 shikimate kinase AroK -
  CSB90_RS03590 (CSB90_0726) aroB 758038..759144 (+) 1107 WP_003135765.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=230883 CSB90_RS03570 WP_003103268.1 754116..754739(+) (pilO) [Pseudomonas aeruginosa strain AR_0353]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=230883 CSB90_RS03570 WP_003103268.1 754116..754739(+) (pilO) [Pseudomonas aeruginosa strain AR_0353]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTCC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401