Detailed information NTG0230319

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CBR65_RS08205
• Genome accession: NZ_CP021382
• Coordinates: 1987273..1988157 (+)
• Length: 294 a.a.
• NCBI ID: WP_087466411.1
• Uniprot ID: A0A1Y0FVC0
• Organism: Cellvibrio sp. PSBB006
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1974908..1987270	1987273..1988157	flank	3

Gene organization within MGE regions

Location: 1974908..1988157

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CBR65_RS08165 (CBR65_08150)	-	1974908..1975456 (-)	549	WP_087466403.1	tetratricopeptide repeat protein	-
CBR65_RS08170 (CBR65_08155)	-	1975472..1976314 (-)	843	WP_157672192.1	RHS repeat domain-containing protein	-
CBR65_RS08175 (CBR65_08160)	-	1976460..1976687 (-)	228	WP_087466405.1	hypothetical protein	-
CBR65_RS08180 (CBR65_08165)	-	1976700..1981223 (-)	4524	WP_198300909.1	RHS repeat-associated core domain-containing protein	-
CBR65_RS08185 (CBR65_08170)	-	1981581..1983323 (-)	1743	WP_087466407.1	PglL family O-oligosaccharyltransferase	-
CBR65_RS08190 (CBR65_08175)	-	1983374..1983883 (-)	510	WP_087466408.1	pilin	-
CBR65_RS08195 (CBR65_08180)	pilB	1984267..1985997 (+)	1731	WP_087466409.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CBR65_RS08200 (CBR65_08185)	pilC	1986002..1987270 (+)	1269	WP_087466410.1	type II secretion system F family protein	Machinery gene
CBR65_RS08205 (CBR65_08190)	pilD	1987273..1988157 (+)	885	WP_087466411.1	A24 family peptidase	Machinery gene

Sequence

Protein

• Length: 294 a.a.
• Molecular weight: 32791.89 Da
• Isoelectric Point: 5.7361

>NTDB_id=230319 CBR65_RS08205 WP_087466411.1 1987273..1988157(+) (pilD) [Cellvibrio sp. PSBB006]
MQDVAQVFALYPKTVTFFLFLIGCVVGSFLNVVIYRLPKMMEAEWRQQCTELLDTSDAATVENSTQFNLAFPSSHCPVCN
HSIRAWENIPVISYLFLRGKCSACKTPISLRYPIIELVTGILSAVVIYFFGLSYLGLAALVFTWCLITLTMIDVDTQLLP
DDITLPLLWLGLIVNSFGLVVPLQDAVWGAIAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLSALGAWMGWQMLPQVILLS
SFVGAALGIAMIVLRGRDKNIPIPFGPYLAIAGWIAFLWGDYINRTYLQLFISP

Nucleotide

• Length: 885 bp

>NTDB_id=230319 CBR65_RS08205 WP_087466411.1 1987273..1988157(+) (pilD) [Cellvibrio sp. PSBB006]
ATGCAAGATGTTGCTCAAGTTTTTGCTCTTTATCCAAAAACGGTTACTTTTTTCTTATTTCTAATTGGCTGTGTCGTCGG
CAGCTTCCTAAACGTCGTCATCTACCGCCTCCCGAAAATGATGGAGGCGGAATGGCGGCAGCAATGTACGGAGTTGCTCG
ATACCAGCGATGCAGCCACTGTAGAAAACTCTACCCAATTCAACCTCGCCTTCCCCTCCTCCCACTGCCCCGTGTGCAAT
CACAGTATTCGTGCTTGGGAGAACATCCCTGTTATCAGCTATCTATTCCTACGTGGTAAATGCTCGGCCTGTAAAACGCC
GATTTCGTTGCGTTATCCAATCATCGAACTGGTTACCGGCATCTTGTCGGCGGTGGTGATTTATTTCTTCGGTTTGAGTT
ATTTGGGGTTGGCAGCGCTAGTGTTTACCTGGTGTCTGATTACGCTGACCATGATTGATGTTGATACACAGTTACTGCCG
GACGATATAACTCTGCCGCTCTTATGGCTAGGCTTGATCGTCAACAGTTTTGGATTGGTTGTGCCCTTGCAGGATGCTGT
TTGGGGTGCGATTGCGGGTTATCTGTCTTTGTGGTCGGTGTATTGGCTGTTTAAGTTGGTAACCGGTAAGGAGGGCATGG
GTTATGGGGATTTCAAATTGCTGAGTGCGCTCGGTGCCTGGATGGGCTGGCAGATGCTGCCGCAGGTTATCCTGCTGTCG
TCCTTTGTAGGGGCTGCGCTGGGCATTGCTATGATTGTGCTGCGAGGGCGGGATAAAAATATTCCCATTCCGTTTGGGCC
TTATCTGGCGATTGCTGGCTGGATTGCGTTTTTGTGGGGTGATTATATTAATCGCACCTACCTGCAACTCTTTATTTCTC
CCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-249)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-129)

3D structure

• AlphaFold DB: A0A1Y0FVC0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	53.819	97.959	0.527
pilD	Acinetobacter nosocomialis M2	53.125	97.959	0.52
pilD	Vibrio campbellii strain DS40M4	53.004	96.259	0.51
pilD	Vibrio cholerae strain A1552	52.65	96.259	0.507
pilD	Neisseria gonorrhoeae MS11	52.83	90.136	0.476