Detailed information NTG0229802

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CBL15_RS14315
• Genome accession: NZ_CP021321
• Coordinates: 2972691..2973809 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain XH731
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2967691..2978809

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CBL15_RS14305 (CBL15_14165)	-	2970807..2971499 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
CBL15_RS14310 (CBL15_14170)	pilT	2971626..2972663 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
CBL15_RS14315 (CBL15_14175)	pilU	2972691..2973809 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CBL15_RS14320 (CBL15_14180)	fur	2973879..2974316 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
CBL15_RS14325 (CBL15_14185)	-	2974428..2974826 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
CBL15_RS14330 (CBL15_14190)	-	2974881..2975204 (-)	324	WP_000442587.1	RnfH family protein	-
CBL15_RS14335 (CBL15_14195)	-	2975204..2976271 (-)	1068	WP_002019572.1	hypothetical protein	-
CBL15_RS14345 (CBL15_14205)	-	2976424..2976879 (-)	456	WP_000782976.1	bacteriohemerythrin	-
CBL15_RS14350 (CBL15_14210)	-	2977132..2977980 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=229802 CBL15_RS14315 WP_000347039.1 2972691..2973809(+) (pilU) [Acinetobacter baumannii strain XH731]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=229802 CBL15_RS14315 WP_000347039.1 2972691..2973809(+) (pilU) [Acinetobacter baumannii strain XH731]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395