Detailed information NTG0227787

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: B6257_RS02575
• Genome accession: NZ_CP021011
• Coordinates: 493943..494557 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain GFP-2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 488943..499557

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B6257_RS02550 (B6257_02530)	rpsT	489258..489524 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
B6257_RS02555 (B6257_02535)	holA	489541..490582 (-)	1042	Protein_513	DNA polymerase III subunit delta	-
B6257_RS19825	-	490622..490776 (-)	155	Protein_514	hypothetical protein	-
B6257_RS02560 (B6257_02540)	-	490817..490951 (+)	135	WP_003152870.1	YqzM family protein	-
B6257_RS02565 (B6257_02545)	comEC	490970..493306 (-)	2337	WP_101562283.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
B6257_RS02570 (B6257_02550)	-	493307..493876 (-)	570	WP_003152868.1	ComE operon protein 2	-
B6257_RS02575 (B6257_02555)	comEA	493943..494557 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
B6257_RS02580 (B6257_02560)	comER	494616..495437 (+)	822	WP_101562284.1	late competence protein ComER	-
B6257_RS02585 (B6257_02565)	-	495506..496243 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
B6257_RS02590 (B6257_02570)	rsfS	496240..496596 (-)	357	WP_003152864.1	ribosome silencing factor	-
B6257_RS02595 (B6257_02575)	yqeK	496614..497174 (-)	561	WP_031378934.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
B6257_RS02600 (B6257_02580)	-	497164..497733 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
B6257_RS02605 (B6257_02585)	yhbY	497744..498034 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
B6257_RS02610 (B6257_02590)	aroE	498028..498870 (-)	843	WP_031378933.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=227787 B6257_RS02575 WP_013352942.1 493943..494557(-) (comEA) [Bacillus velezensis strain GFP-2]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=227787 B6257_RS02575 WP_013352942.1 493943..494557(-) (comEA) [Bacillus velezensis strain GFP-2]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCCTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363