Detailed information NTG0227785

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: B6257_RS02565
• Genome accession: NZ_CP021011
• Coordinates: 490970..493306 (-)
• Length: 778 a.a.
• NCBI ID: WP_101562283.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain GFP-2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 485970..498306

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B6257_RS02535 (B6257_02515)	-	486333..486671 (-)	339	WP_007408270.1	YqxA family protein	-
B6257_RS02540 (B6257_02520)	spoIIP	486688..487881 (-)	1194	WP_063174745.1	stage II sporulation protein P	-
B6257_RS02545 (B6257_02525)	gpr	487949..489055 (-)	1107	WP_003152878.1	GPR endopeptidase	-
B6257_RS02550 (B6257_02530)	rpsT	489258..489524 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
B6257_RS02555 (B6257_02535)	holA	489541..490582 (-)	1042	Protein_513	DNA polymerase III subunit delta	-
B6257_RS19825	-	490622..490776 (-)	155	Protein_514	hypothetical protein	-
B6257_RS02560 (B6257_02540)	-	490817..490951 (+)	135	WP_003152870.1	YqzM family protein	-
B6257_RS02565 (B6257_02545)	comEC	490970..493306 (-)	2337	WP_101562283.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
B6257_RS02570 (B6257_02550)	-	493307..493876 (-)	570	WP_003152868.1	ComE operon protein 2	-
B6257_RS02575 (B6257_02555)	comEA	493943..494557 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
B6257_RS02580 (B6257_02560)	comER	494616..495437 (+)	822	WP_101562284.1	late competence protein ComER	-
B6257_RS02585 (B6257_02565)	-	495506..496243 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
B6257_RS02590 (B6257_02570)	rsfS	496240..496596 (-)	357	WP_003152864.1	ribosome silencing factor	-
B6257_RS02595 (B6257_02575)	yqeK	496614..497174 (-)	561	WP_031378934.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
B6257_RS02600 (B6257_02580)	-	497164..497733 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
B6257_RS02605 (B6257_02585)	yhbY	497744..498034 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 778 a.a.
• Molecular weight: 86113.95 Da
• Isoelectric Point: 8.8228

>NTDB_id=227785 B6257_RS02565 WP_101562283.1 490970..493306(-) (comEC) [Bacillus velezensis strain GFP-2]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQPAPVIICLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRRIEQLEPGMRCTFTGSLEQPAHATIPGGFDYKEYLYSQQI
HWLFSVTSIQRCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGMLLLLFLPVYTLLSGAASSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPYRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQCQVLSPESSDG
KSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGKENRYH
HPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2337 bp

>NTDB_id=227785 B6257_RS02565 WP_101562283.1 490970..493306(-) (comEC) [Bacillus velezensis strain GFP-2]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCCTGCTCCGGTTATCATCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTATAAAATCCGGTCTCTTGAGGAAAAGAGACGCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGATTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCGGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCAATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCAGGAATGCATGTC
GGTCTTATTACGGCGGGACTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGATGTTGCTGCTGCT
GTTTTTGCCGGTATATACGCTGCTGAGCGGTGCCGCCTCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCGGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCCTCATTCATTGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGTCAGTTTTCCGATGAACATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGCTTCCTTCTTCTTTTGCTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAGCGCGCCATACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCACATCGGGGAAGCCGGAGTGTTAATCAAACG
GTTAATTGTCCCTGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAGAAAACAACATTCCCG
TTGCCGAAGCAAAGCGGGGCGACACCATTACAGCGGGTGATCTTCAGTGTCAGGTGCTGTCTCCGGAGTCATCTGACGGA
AAGAGTAAAAATGATTCATCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGACGGGAGATTTAGAATC
GGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTCATCACGGCAGCAAAA
GCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAAGAGAATCGATACCAT
CATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATATCAGCGGAACGATTCA
ATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCAGGGCGCAAGAAGTAA
AAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-714)

• Competence protein (Competence) (208-469)

• Domain of unknown function (DUF4131) (DUF4131) (25-170)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	55.699	99.229	0.553