Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   B6257_RS02155 Genome accession   NZ_CP021011
Coordinates   417453..418523 (-) Length   356 a.a.
NCBI ID   WP_070082113.1    Uniprot ID   -
Organism   Bacillus velezensis strain GFP-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412453..423523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6257_RS02105 (B6257_02090) sipW 412513..413097 (-) 585 WP_101562270.1 signal peptidase I SipW -
  B6257_RS02110 (B6257_02095) tapA 413069..413740 (-) 672 WP_101562271.1 amyloid fiber anchoring/assembly protein TapA -
  B6257_RS02115 (B6257_02100) - 413999..414328 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  B6257_RS02120 (B6257_02105) - 414368..414547 (-) 180 WP_003153093.1 YqzE family protein -
  B6257_RS02125 (B6257_02110) comGG 414604..414981 (-) 378 WP_101562272.1 competence type IV pilus minor pilin ComGG Machinery gene
  B6257_RS02130 (B6257_02115) comGF 414982..415482 (-) 501 WP_014305411.1 competence type IV pilus minor pilin ComGF -
  B6257_RS02135 (B6257_02120) comGE 415391..415705 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  B6257_RS02140 (B6257_02125) comGD 415689..416126 (-) 438 WP_025852922.1 competence type IV pilus minor pilin ComGD Machinery gene
  B6257_RS02145 (B6257_02130) comGC 416116..416382 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  B6257_RS02150 (B6257_02135) comGB 416429..417466 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  B6257_RS02155 (B6257_02140) comGA 417453..418523 (-) 1071 WP_070082113.1 competence type IV pilus ATPase ComGA Machinery gene
  B6257_RS02160 (B6257_02145) - 418714..419664 (-) 951 WP_071391609.1 magnesium transporter CorA family protein -
  B6257_RS02165 (B6257_02150) - 419810..421111 (+) 1302 WP_070082114.1 hemolysin family protein -
  B6257_RS02170 (B6257_02155) - 421149..421979 (-) 831 WP_071391608.1 STAS domain-containing protein -
  B6257_RS02180 (B6257_02165) - 422184..422564 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  B6257_RS02185 (B6257_02170) - 422801..423046 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.91 Da        Isoelectric Point: 9.5862

>NTDB_id=227783 B6257_RS02155 WP_070082113.1 417453..418523(-) (comGA) [Bacillus velezensis strain GFP-2]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFREAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=227783 B6257_RS02155 WP_070082113.1 417453..418523(-) (comGA) [Bacillus velezensis strain GFP-2]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTCTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTTACCCAATGCTTTCGGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTAATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment