Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XcfCFBP6975P_RS06870 Genome accession   NZ_CP021006
Coordinates   1580304..1582040 (+) Length   578 a.a.
NCBI ID   WP_022558667.1    Uniprot ID   -
Organism   Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6975     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1575304..1587040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XcfCFBP6975P_RS06845 (XcfCFBP6975P_17395) coaE 1576216..1576824 (-) 609 WP_022558665.1 dephospho-CoA kinase -
  XcfCFBP6975P_RS06850 (XcfCFBP6975P_17400) - 1576838..1577701 (-) 864 WP_022558666.1 A24 family peptidase -
  XcfCFBP6975P_RS06855 (XcfCFBP6975P_17405) pilC 1577708..1578964 (-) 1257 WP_007973903.1 type II secretion system F family protein Machinery gene
  XcfCFBP6975P_RS06860 (XcfCFBP6975P_17410) pilE 1579291..1579731 (+) 441 WP_005934296.1 pilin Machinery gene
  XcfCFBP6975P_RS06865 (XcfCFBP6975P_17415) - 1579840..1580241 (+) 402 WP_046121195.1 pilin -
  XcfCFBP6975P_RS06870 (XcfCFBP6975P_17420) pilB 1580304..1582040 (+) 1737 WP_022558667.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XcfCFBP6975P_RS06875 (XcfCFBP6975P_17430) pilR 1582490..1583944 (-) 1455 WP_022558668.1 sigma-54 dependent transcriptional regulator Regulator
  XcfCFBP6975P_RS06880 (XcfCFBP6975P_17435) - 1584214..1585827 (-) 1614 WP_007965357.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62578.67 Da        Isoelectric Point: 5.6006

>NTDB_id=227760 XcfCFBP6975P_RS06870 WP_022558667.1 1580304..1582040(+) (pilB) [Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6975]
MSVLSTANLVGITGIARRLVQDGALEESAARSAMDQAALAKVPLPQWFSEKKLVAASQLAAANAVEFGMPLLDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=227760 XcfCFBP6975P_RS06870 WP_022558667.1 1580304..1582040(+) (pilB) [Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6975]
ATGAGCGTTTTATCGACCGCAAATCTCGTTGGAATCACCGGAATTGCCCGTAGGCTGGTGCAGGATGGCGCACTGGAGGA
AAGCGCGGCTCGAAGCGCGATGGATCAGGCTGCTCTTGCGAAAGTTCCACTGCCTCAGTGGTTCTCGGAGAAAAAGCTCG
TCGCTGCCTCGCAGTTGGCAGCAGCCAACGCAGTCGAGTTCGGCATGCCGCTGCTCGATGTTTCTGCATTCGACGCCAGC
CAGAACGCAGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAAGCCA
TTCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCTAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAGATGGGGGATCTGGACGTTTCGGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCGTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAATGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AAGTGGTGCTGCGTATCCTGGACGGCAGTGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCAGACCAGCAGAAG
TTGTTCCTGGAGGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTCGCGGCAGCCTTGCGCTCGTTCCTGCGACAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCCCGTTTGATGAACATGGGCATTGCGCCCTACAACATCA
CCTCGTCAGTGACGCTGGTGATTGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTTCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGC
TGGAGGGCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGGCAATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCGGAGATCAATCGAGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.959

100

0.561
  pilB Acinetobacter baylyi ADP1

54.75

100

0.548
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

48.873

99.827

0.488
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

96.886

0.455
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45

Multiple sequence alignment