Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B9J09_RS10840 Genome accession   NZ_CP020870
Coordinates   2249871..2251604 (+) Length   577 a.a.
NCBI ID   WP_046418464.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. pauca strain De Donno     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2244871..2256604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9J09_RS10815 (B9J09_10815) - 2245214..2246405 (-) 1192 Protein_2023 type II secretion system F family protein -
  B9J09_RS10820 (B9J09_10820) - 2246854..2247300 (+) 447 WP_010894960.1 pilin -
  B9J09_RS10830 (B9J09_10830) - 2248596..2249033 (+) 438 WP_046418469.1 pilin -
  B9J09_RS10840 (B9J09_10840) pilB 2249871..2251604 (+) 1734 WP_046418464.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B9J09_RS10845 (B9J09_10845) pilR 2251698..2253110 (-) 1413 WP_046418462.1 sigma-54 dependent transcriptional regulator Regulator
  B9J09_RS10850 (B9J09_10850) - 2253296..2254906 (-) 1611 WP_046418460.1 ATP-binding protein -
  B9J09_RS10855 (B9J09_10855) sucC 2255155..2256318 (+) 1164 WP_046418841.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63010.09 Da        Isoelectric Point: 5.9154

>NTDB_id=226705 B9J09_RS10840 WP_046418464.1 2249871..2251604(+) (pilB) [Xylella fastidiosa subsp. pauca strain De Donno]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSTAKLAAANALEFGMPLLDVSAFDHN
HNAMSLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALE
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGMTGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=226705 B9J09_RS10840 WP_046418464.1 2249871..2251604(+) (pilB) [Xylella fastidiosa subsp. pauca strain De Donno]
ATGAACACACCTCATTCAGCCAATCTTGTCGGCATCACCGGCATTGCCCGTCGCCTAGTCCAGGATGGCAGCATCGAGGA
AGCCACCGCACGCACTGCTCAAAATCAAGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TAAGTACCGCAAAGTTGGCCGCAGCCAATGCACTTGAGTTCGGGATGCCGCTGCTGGATGTCTCCGCATTCGACCACAAT
CACAATGCAATGAGCTTGGTCGACGAAGAATTACTGCAAAAGCATCGGGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACCCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGACGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAG
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACCGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCGTCAATAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATTGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTACTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTACTGGTCACTGGTCCGACCGGTTCGGGCAAGACAGTGTCACTGTA
CACTGCACTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGTG
TCAATCAGGTACAACAAAACAACAAGCGCGGTATGACGTTTGCCGCAGCATTACGTTCGTTTCTGCGCCAAGACCCGGAT
GTCATCATGGTCGGCGAAATCCGTGATCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACCCTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAACAAGTGCAAGCGCCCGGTTCAATTGCCGACA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACCGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGTGGTAATGCCTTACAAATCGCCGAAGAGGCACAGCGCATCGGTATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGTATGACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.143

98.267

0.562
  pilB Acinetobacter baumannii D1279779

56.437

98.267

0.555
  pilB Legionella pneumophila strain ERS1305867

51.585

98.44

0.508
  pilF Neisseria gonorrhoeae MS11

50.264

98.614

0.496
  pilB Vibrio cholerae strain A1552

49.54

94.107

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

46.903

97.92

0.459
  pilB Vibrio campbellii strain DS40M4

46.561

98.267

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.993

92.894

0.362

Multiple sequence alignment