Detailed information NTG0226699

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: B9J09_RS06695
• Genome accession: NZ_CP020870
• Coordinates: 1351538..1352668 (+)
• Length: 376 a.a.
• NCBI ID: WP_010894128.1
• Uniprot ID: Q9PCX2
• Organism: Xylella fastidiosa subsp. pauca strain De Donno
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1346538..1357668

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B9J09_RS06670 (B9J09_06670)	-	1346761..1347585 (+)	825	WP_010894133.1	glycosyltransferase family 2 protein	-
B9J09_RS06680 (B9J09_06680)	-	1348249..1349076 (-)	828	WP_171817080.1	glycosyltransferase family 2 protein	-
B9J09_RS06685 (B9J09_06685)	-	1349489..1349701 (-)	213	WP_046419721.1	hypothetical protein	-
B9J09_RS06690 (B9J09_06690)	pilT	1350338..1351372 (+)	1035	WP_046419718.1	type IV pilus twitching motility protein PilT	Machinery gene
B9J09_RS06695 (B9J09_06695)	pilU	1351538..1352668 (+)	1131	WP_010894128.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B9J09_RS06700 (B9J09_06700)	-	1353185..1354198 (-)	1014	WP_046419716.1	YafY family protein	-
B9J09_RS06710 (B9J09_06710)	-	1355042..1355851 (+)	810	WP_046419714.1	DUF481 domain-containing protein	-
B9J09_RS06715 (B9J09_06715)	hemC	1355905..1356822 (-)	918	WP_046419711.1	hydroxymethylbilane synthase	-
B9J09_RS06720 (B9J09_06720)	-	1356839..1357597 (-)	759	WP_010894122.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42476.67 Da
• Isoelectric Point: 6.5027

>NTDB_id=226699 B9J09_RS06695 WP_010894128.1 1351538..1352668(+) (pilU) [Xylella fastidiosa subsp. pauca strain De Donno]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITN
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLTDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=226699 B9J09_RS06695 WP_010894128.1 1351538..1352668(+) (pilU) [Xylella fastidiosa subsp. pauca strain De Donno]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAAGTCCATGAATGCAACTTCGCAATTGACATCACCAAC
ATTGGTCGCTTCCGCGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCAGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGACTGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACTACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCCAAAACTCTGAGTCAAGGATTCGATGGAGTAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-272)

3D structure

• AlphaFold DB: Q9PCX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	51.124	94.681	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399