Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   B7941_RS14570 Genome accession   NZ_CP020805
Coordinates   2973135..2974205 (-) Length   356 a.a.
NCBI ID   WP_085342191.1    Uniprot ID   -
Organism   Bacillus velezensis strain 9D-6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2968135..2979205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7941_RS14520 (B7941_14505) sipW 2968210..2968779 (-) 570 WP_085342188.1 signal peptidase I SipW -
  B7941_RS14525 (B7941_14510) tapA 2968751..2969422 (-) 672 WP_085342189.1 amyloid fiber anchoring/assembly protein TapA -
  B7941_RS14530 (B7941_14515) - 2969681..2970010 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  B7941_RS14535 (B7941_14520) - 2970050..2970229 (-) 180 WP_003153093.1 YqzE family protein -
  B7941_RS14540 (B7941_14525) comGG 2970286..2970663 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  B7941_RS14545 (B7941_14530) comGF 2970664..2971164 (-) 501 WP_257474763.1 competence type IV pilus minor pilin ComGF -
  B7941_RS14550 (B7941_14535) comGE 2971073..2971387 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  B7941_RS14555 (B7941_14540) comGD 2971371..2971808 (-) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  B7941_RS14560 (B7941_14545) comGC 2971798..2972106 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  B7941_RS14565 (B7941_14550) comGB 2972111..2973148 (-) 1038 WP_085342190.1 competence type IV pilus assembly protein ComGB Machinery gene
  B7941_RS14570 (B7941_14555) comGA 2973135..2974205 (-) 1071 WP_085342191.1 competence type IV pilus ATPase ComGA Machinery gene
  B7941_RS14575 (B7941_14560) - 2974402..2975352 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  B7941_RS14580 (B7941_14565) - 2975498..2976799 (+) 1302 WP_012117986.1 hemolysin family protein -
  B7941_RS14585 (B7941_14570) - 2976837..2977667 (-) 831 WP_015240214.1 STAS domain-containing protein -
  B7941_RS14595 (B7941_14580) - 2977874..2978254 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  B7941_RS14600 (B7941_14585) - 2978492..2978737 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40587.89 Da        Isoelectric Point: 9.3485

>NTDB_id=225963 B7941_RS14570 WP_085342191.1 2973135..2974205(-) (comGA) [Bacillus velezensis strain 9D-6]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKWQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=225963 B7941_RS14570 WP_085342191.1 2973135..2974205(-) (comGA) [Bacillus velezensis strain 9D-6]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGTGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTCTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment