Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   B5D77_RS20530 Genome accession   NZ_CP020664
Coordinates   4026654..4028669 (-) Length   671 a.a.
NCBI ID   WP_008196489.1    Uniprot ID   A0A2P1UK99
Organism   Microcystis sp. MC19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4021654..4033669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5D77_RS20520 (B5D77_20270) - 4023985..4025199 (-) 1215 WP_008196494.1 type II secretion system F family protein -
  B5D77_RS25980 - 4025132..4025302 (+) 171 WP_159036980.1 hypothetical protein -
  B5D77_RS20525 (B5D77_20275) pilT 4025401..4026501 (-) 1101 WP_008196492.1 type IV pilus twitching motility protein PilT Machinery gene
  B5D77_RS20530 (B5D77_20280) pilB/pilB1 4026654..4028669 (-) 2016 WP_008196489.1 GspE/PulE family protein Machinery gene
  B5D77_RS20535 (B5D77_20285) grpE 4028910..4029632 (+) 723 WP_106909549.1 nucleotide exchange factor GrpE -
  B5D77_RS20540 (B5D77_20290) - 4029774..4030433 (-) 660 WP_106909550.1 HAD-IA family hydrolase -
  B5D77_RS20545 (B5D77_20295) - 4030524..4032488 (-) 1965 WP_106909551.1 amylo-alpha-1,6-glucosidase -
  B5D77_RS20550 (B5D77_20300) rpmH 4032579..4032716 (+) 138 WP_002739276.1 50S ribosomal protein L34 -
  B5D77_RS20555 (B5D77_20305) rnpA 4032743..4033105 (+) 363 WP_106909552.1 ribonuclease P protein component -
  B5D77_RS20560 (B5D77_20310) - 4033095..4033490 (+) 396 WP_002763099.1 PH domain-containing protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75016.97 Da        Isoelectric Point: 5.7255

>NTDB_id=225350 B5D77_RS20530 WP_008196489.1 4026654..4028669(-) (pilB/pilB1) [Microcystis sp. MC19]
MTFSAKKTRAVALRNYFSPFGNKLVQSGYIGADQMQQALVETRKSGRSLVEILQQLTGRPLPPDLQRQYKKNQLFELKIL
YGVESIDPELTDVDEMEIARLIDSLIPIDLCRRYRLIPLRKIEGEPAAILIAMVDPDNLEASDDINRILRPRDLGLQRLV
ITGEDYERLLEKFHRAQSELDQEKARLQKEKELEKLSDLTDIVGNIEVASSAANDEAADDLGEGEANQAPVINLVNKILA
KALQEGSSDIHIEPQEEFLKIRFRKDGVLHQAFDPLPKKITPAVTARFKIMADLDIAERRLPQDGKIRRIYQGRKVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDQKTLQIVRDMASRPFGLLLVTGPTGSGKSTTLYSVLAERNYPGVNINTA
EDPIEYSLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLD
EMGVEPFMISGSLLGVLAQRLVRRVCTECRVAYHPGQGELARFGLAAANEREVTFYKANTLTLEEIQQARKQGNLCPKCS
GSGYKGRVGVYEVMQNSERLQQLINQGATTDRIKEAAVDEGMVTLLAYSLNLVREGYTTLEEVERVTFTDSGLESELKAK
RKSGLICRGCRAELQPEWLDCPYCMTPRFSD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=225350 B5D77_RS20530 WP_008196489.1 4026654..4028669(-) (pilB/pilB1) [Microcystis sp. MC19]
ATGACATTTTCTGCCAAGAAAACCCGTGCCGTTGCCCTACGCAATTACTTTTCCCCCTTTGGCAACAAGTTGGTGCAGTC
GGGCTATATCGGTGCCGACCAAATGCAACAGGCCCTAGTGGAAACACGGAAATCGGGACGATCGCTAGTAGAAATATTAC
AACAACTAACCGGGCGCCCTTTGCCCCCAGATTTGCAGCGACAGTATAAGAAAAATCAGTTATTTGAATTAAAAATTCTC
TACGGAGTCGAATCGATCGACCCCGAATTAACCGATGTGGATGAAATGGAAATAGCGCGATTGATCGACTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGCGAAAGATCGAAGGGGAGCCGGCTGCGATTTTGATAGCCATGG
TCGATCCCGACAACCTCGAGGCTTCCGACGATATTAACCGCATTCTCCGCCCCAGGGATTTAGGTTTACAGCGTTTGGTG
ATTACCGGCGAGGACTATGAAAGATTACTGGAAAAATTCCATCGGGCCCAATCGGAACTAGACCAGGAAAAAGCCCGACT
GCAAAAAGAAAAAGAACTGGAAAAACTCTCGGATCTGACCGATATTGTCGGCAATATTGAGGTGGCCTCCAGTGCGGCTA
ACGATGAAGCGGCCGATGATCTCGGGGAAGGGGAGGCTAATCAGGCCCCCGTCATCAACCTGGTCAATAAAATTCTAGCT
AAGGCCCTACAGGAAGGATCCTCGGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTCATCAAGCTTTTGATCCACTGCCGAAAAAAATTACCCCGGCGGTCACTGCCCGTTTTAAAATCATGGCCGATC
TCGATATCGCCGAACGTCGTCTTCCCCAAGATGGTAAAATTCGGCGGATCTATCAAGGGCGGAAAGTGGATTTTCGGGTT
AATACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTACGGATTCTCGACAACTCGGCCACCCAGTTGGGTTTAGATAA
ACTAATCACCGATCAAAAAACCCTGCAAATCGTCCGAGATATGGCTAGTCGTCCCTTTGGTCTGTTGCTGGTGACAGGCC
CGACCGGTTCGGGGAAATCCACCACTTTATACTCGGTCTTAGCGGAAAGAAATTACCCCGGAGTTAATATTAATACCGCC
GAGGACCCGATCGAATATTCTCTACCGGGTATCACCCAGGTGCAGGTAATTCGGGAAAAGGGCATGGATTTCGCCTCGAT
TCTCCGGGCCTTCCTGCGACAAGATCCGGATGTGATTCTGGTGGGAGAAACTAGGGATAAGGAAACGGCTAAAACAGCGA
TCGAGGCCGCCCTAACCGGTCACTTGGTCCTAACTACCCTACACACCAACGATGCCGCAGGTGCGATCGCTCGTCTCGAC
GAGATGGGGGTTGAACCGTTTATGATTTCTGGTTCCCTCTTGGGAGTCTTGGCCCAGCGTCTCGTGCGCCGCGTCTGTAC
TGAGTGCCGAGTCGCCTATCACCCCGGTCAAGGGGAATTGGCCCGTTTCGGTCTTGCCGCGGCCAATGAACGGGAAGTGA
CCTTTTATAAGGCTAATACCCTGACCCTAGAGGAAATTCAACAGGCGCGAAAACAGGGTAATCTTTGTCCCAAATGTAGC
GGCAGCGGTTATAAAGGTCGCGTCGGGGTCTATGAGGTAATGCAGAATAGTGAACGACTGCAGCAGTTAATTAACCAAGG
GGCAACCACCGATCGCATTAAGGAGGCGGCCGTCGACGAGGGCATGGTGACACTCCTCGCCTATAGTTTAAATCTGGTGC
GCGAGGGTTACACCACCCTCGAAGAGGTGGAACGGGTGACATTTACCGATTCTGGTCTGGAATCGGAACTAAAAGCCAAA
CGTAAAAGCGGTTTAATCTGTCGTGGTTGTCGCGCCGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGACACC
GCGATTTAGTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-179)

Type II/IV secretion system protein (T2SSE)

(237-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2P1UK99

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

73.716

98.659

0.727
  pilF Thermus thermophilus HB27

40.164

90.909

0.365

Multiple sequence alignment