Detailed information NTG0224741

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: B7L41_RS06465
• Genome accession: NZ_CP020595
• Coordinates: 1215374..1216492 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain USA15
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1210374..1221492

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L41_RS06430 (B7L41_06430)	-	1211203..1212051 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
B7L41_RS06435 (B7L41_06435)	-	1212304..1212759 (+)	456	WP_000782976.1	bacteriohemerythrin	-
B7L41_RS06445 (B7L41_06445)	-	1212912..1213979 (+)	1068	WP_000092403.1	hypothetical protein	-
B7L41_RS06450 (B7L41_06450)	-	1213979..1214302 (+)	324	WP_000442585.1	RnfH family protein	-
B7L41_RS06455 (B7L41_06455)	-	1214357..1214755 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
B7L41_RS06460 (B7L41_06460)	fur	1214867..1215304 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
B7L41_RS06465 (B7L41_06465)	pilU	1215374..1216492 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B7L41_RS06470 (B7L41_06470)	pilT	1216520..1217557 (-)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
B7L41_RS06475 (B7L41_06475)	-	1217684..1218376 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=224741 B7L41_RS06465 WP_000347036.1 1215374..1216492(-) (pilU) [Acinetobacter baumannii strain USA15]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=224741 B7L41_RS06465 WP_000347036.1 1215374..1216492(-) (pilU) [Acinetobacter baumannii strain USA15]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392