Detailed information NTG0224703

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: B7L39_RS12520
• Genome accession: NZ_CP020592
• Coordinates: 2633383..2634441 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain USA2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2628383..2639441

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L39_RS12495 (B7L39_12495)	aroK	2628653..2629222 (-)	570	WP_000643712.1	shikimate kinase AroK	-
B7L39_RS12500 (B7L39_12500)	pilQ	2629234..2631399 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
B7L39_RS12505 (B7L39_12505)	pilP	2631467..2631994 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
B7L39_RS12510 (B7L39_12510)	pilO	2632005..2632745 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
B7L39_RS12515 (B7L39_12515)	pilN	2632742..2633383 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
B7L39_RS12520 (B7L39_12520)	pilM	2633383..2634441 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
B7L39_RS12525 (B7L39_12525)	ponA	2634604..2637159 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
B7L39_RS12530 (B7L39_12530)	-	2637177..2638001 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
B7L39_RS12535 (B7L39_12535)	-	2638004..2638336 (-)	333	WP_000169594.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=224703 B7L39_RS12520 WP_002014066.1 2633383..2634441(-) (pilM) [Acinetobacter baumannii strain USA2]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=224703 B7L39_RS12520 WP_002014066.1 2633383..2634441(-) (pilM) [Acinetobacter baumannii strain USA2]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423