Detailed information NTG0224563

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: B7L44_RS16080
• Genome accession: NZ_CP020588
• Coordinates: 3295564..3296682 (+)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain SSA3
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3290564..3301682

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L44_RS16065 (B7L44_16065)	-	3292730..3293572 (-)	843	WP_006581385.1	undecaprenyl-diphosphate phosphatase	-
B7L44_RS16070 (B7L44_16070)	-	3293680..3294372 (-)	693	WP_016804433.1	YggS family pyridoxal phosphate-dependent enzyme	-
B7L44_RS16075 (B7L44_16075)	pilT	3294499..3295536 (+)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
B7L44_RS16080 (B7L44_16080)	pilU	3295564..3296682 (+)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B7L44_RS16085 (B7L44_16085)	fur	3296752..3297189 (-)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
B7L44_RS16090 (B7L44_16090)	-	3297301..3297699 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
B7L44_RS16095 (B7L44_16095)	-	3297755..3298078 (-)	324	WP_002052062.1	RnfH family protein	-
B7L44_RS16100 (B7L44_16100)	-	3298078..3299145 (-)	1068	WP_004710227.1	hypothetical protein	-
B7L44_RS16110 (B7L44_16110)	-	3299298..3299753 (-)	456	WP_000782976.1	bacteriohemerythrin	-
B7L44_RS16115 (B7L44_16115)	-	3300006..3300854 (-)	849	WP_016804434.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=224563 B7L44_RS16080 WP_002052019.1 3295564..3296682(+) (pilU) [Acinetobacter nosocomialis strain SSA3]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=224563 B7L44_RS16080 WP_002052019.1 3295564..3296682(+) (pilU) [Acinetobacter nosocomialis strain SSA3]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTAATTGGTCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAACGTGAAGTCGGTATTGATACGGACTCTTTCGA
AATTGCTTTAAAAAATACATTACGACAAGCACCAGATGTGATCTTAATTGGTGAAATCCGCTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACAATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
GCAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTCGACTTATATAAAGCGGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAGCTTGCAAATGAAGGTCCTGATCAAATTACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403