Detailed information NTG0224527

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: B7L36_RS17810
• Genome accession: NZ_CP020586
• Coordinates: 3514537..3515655 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain CBA7
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3509537..3520655

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L36_RS17800 (B7L36_17805)	-	3512653..3513345 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
B7L36_RS17805 (B7L36_17810)	pilT	3513472..3514509 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
B7L36_RS17810 (B7L36_17815)	pilU	3514537..3515655 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B7L36_RS17815 (B7L36_17820)	fur	3515725..3516162 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
B7L36_RS17820 (B7L36_17825)	-	3516274..3516672 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
B7L36_RS17825 (B7L36_17830)	-	3516727..3517050 (-)	324	WP_000442587.1	RnfH family protein	-
B7L36_RS17830 (B7L36_17835)	-	3517050..3518117 (-)	1068	WP_002019572.1	hypothetical protein	-
B7L36_RS17840 (B7L36_17845)	-	3518270..3518725 (-)	456	WP_000782976.1	bacteriohemerythrin	-
B7L36_RS17845 (B7L36_17850)	-	3518978..3519826 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=224527 B7L36_RS17810 WP_000347039.1 3514537..3515655(+) (pilU) [Acinetobacter baumannii strain CBA7]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=224527 B7L36_RS17810 WP_000347039.1 3514537..3515655(+) (pilU) [Acinetobacter baumannii strain CBA7]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395