Detailed information NTG0224421

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: B7L43_RS13575
• Genome accession: NZ_CP020581
• Coordinates: 2729173..2730231 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain SSMA17
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2724173..2735231

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L43_RS13560 (B7L43_13560)	-	2725278..2725610 (+)	333	WP_000169594.1	hypothetical protein	-
B7L43_RS13565 (B7L43_13565)	-	2725613..2726437 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
B7L43_RS13570 (B7L43_13570)	ponA	2726455..2729010 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
B7L43_RS13575 (B7L43_13575)	pilM	2729173..2730231 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
B7L43_RS13580 (B7L43_13580)	pilN	2730231..2730872 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
B7L43_RS13585 (B7L43_13585)	pilO	2730869..2731609 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
B7L43_RS13590 (B7L43_13590)	pilP	2731620..2732147 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
B7L43_RS13595 (B7L43_13595)	pilQ	2732215..2734377 (+)	2163	WP_033503048.1	type IV pilus secretin PilQ family protein	Machinery gene
B7L43_RS13600 (B7L43_13600)	aroK	2734389..2734958 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=224421 B7L43_RS13575 WP_002014066.1 2729173..2730231(+) (pilM) [Acinetobacter baumannii strain SSMA17]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=224421 B7L43_RS13575 WP_002014066.1 2729173..2730231(+) (pilM) [Acinetobacter baumannii strain SSMA17]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423