Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A6J88_RS18965 Genome accession   NZ_CP020452
Coordinates   972095..974230 (+) Length   711 a.a.
NCBI ID   WP_080613700.1    Uniprot ID   -
Organism   Neisseria mucosa strain FDAARGOS_260     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 970380..990848 972095..974230 within 0


Gene organization within MGE regions


Location: 970380..990848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J88_RS18955 (A6J88_04885) - 970818..971504 (+) 687 WP_080613699.1 type 4a pilus biogenesis protein PilO -
  A6J88_RS18960 (A6J88_04890) pilP 971522..972076 (+) 555 WP_060974925.1 pilus assembly protein PilP Machinery gene
  A6J88_RS18965 (A6J88_04895) pilQ 972095..974230 (+) 2136 WP_080613700.1 type IV pilus secretin PilQ Machinery gene
  A6J88_RS18970 (A6J88_04900) - 974397..975263 (+) 867 Protein_908 IS630 family transposase -
  A6J88_RS18975 (A6J88_04905) - 975456..975872 (+) 417 WP_060974909.1 DNA modification methylase -
  A6J88_RS18980 (A6J88_04910) - 975970..976578 (+) 609 Protein_910 transposase -
  A6J88_RS28910 (A6J88_04915) - 976581..977353 (-) 773 Protein_911 IS630 family transposase -
  A6J88_RS18995 (A6J88_04925) - 977369..978175 (-) 807 WP_080613701.1 integrase core domain-containing protein -
  A6J88_RS19000 (A6J88_14000) - 978224..978313 (-) 90 Protein_913 IS630 transposase-related protein -
  A6J88_RS19005 (A6J88_04930) - 978364..978765 (+) 402 Protein_914 transposase -
  A6J88_RS19010 (A6J88_04935) - 979278..979814 (+) 537 WP_240594041.1 shikimate kinase -
  A6J88_RS19015 (A6J88_04940) aroB 980015..981094 (+) 1080 WP_080613703.1 3-dehydroquinate synthase -
  A6J88_RS19020 (A6J88_04945) argC 981191..982234 (-) 1044 WP_070440277.1 N-acetyl-gamma-glutamyl-phosphate reductase -
  A6J88_RS19025 (A6J88_04950) - 982518..983516 (-) 999 WP_070440279.1 L,D-transpeptidase -
  A6J88_RS19030 (A6J88_04955) rlmD 983643..984989 (+) 1347 WP_240594043.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  A6J88_RS19035 (A6J88_04960) - 985135..986586 (+) 1452 WP_039404568.1 tetratricopeptide repeat protein -
  A6J88_RS19040 (A6J88_04965) - 986611..987702 (+) 1092 WP_039404565.1 alpha-glucan phosphorylase -
  A6J88_RS19045 (A6J88_04970) - 987715..988251 (+) 537 WP_003743737.1 GTP-binding protein -
  A6J88_RS19050 (A6J88_04975) - 988257..988898 (+) 642 WP_039404562.1 hypothetical protein -
  A6J88_RS19055 (A6J88_04980) - 988938..989297 (+) 360 WP_003743735.1 roadblock/LC7 domain-containing protein -
  A6J88_RS19065 (A6J88_04990) prmC 990021..990848 (+) 828 WP_080614483.1 peptide chain release factor N(5)-glutamine methyltransferase -

Sequence

Protein


Download         Length: 711 a.a.        Molecular weight: 77650.85 Da        Isoelectric Point: 9.7158

>NTDB_id=223298 A6J88_RS18965 WP_080613700.1 972095..974230(+) (pilQ) [Neisseria mucosa strain FDAARGOS_260]
MKTKNMTKLFAGISMIAAAQIALAGNITDINVSTLPDSQKIIKIRFDRDVISPSGFVTSTPSRIALDFTKTNIQLAQPVL
EYADPLLNQITAAQNNDRSRIVLGLNKAAQYNTEVHGNEVWVFVTESSDRTTTSTAISNNANARQKAGSSEAVKQTVNSA
NIDFRKGAHNSGIVELNAPDFTGKPEVKQQRDRVIITLKNYPLPTQAQRSLDVADFSTPVKNITLKRIGNDAQLIIRNNG
NWDFNTKAANGQFAFEVMPKSASTESSGLNANPNKSFRGRKISLDFQDVEVRTILQILAKESGVNIVASDSVNGKMTLSL
KDVPWDQALDLVMQARNLDMRRQGNIINIAPRDELLAKDKAFLQAEKEIAELGPLYSQTFQLKYKNVEEFRKILRLDDNN
SGNSNGRNTLLSGRGSALIDPATNTLIITDNRNVVEKFRKLIEELDVPTRQVMVEARIVEAKDGFSRDLGVKFGVAGSKG
RNAWGNSWSNAQDNHNTNSAVLRGDANYRTWSLQPNISLPAAAATNNIALVRALSSGALGLEISASEATGKSKTISNPRV
LTQDRREATIESGTEIPYQEASSSGATSVSFKKAVLGLTVTPNVTPDGQVIMTVKITKDTPQDCTVDSLITKCIQTKNLN
TQAMVEDGGTLIVGGIYEEDNSDTINKVPLLGDLPVVGNLFKSRAKKEERRELLVFITPRIMDGVGSNLRY

Nucleotide


Download         Length: 2136 bp        

>NTDB_id=223298 A6J88_RS18965 WP_080613700.1 972095..974230(+) (pilQ) [Neisseria mucosa strain FDAARGOS_260]
ATGAAAACTAAGAATATGACGAAATTATTTGCCGGCATCAGTATGATTGCAGCAGCACAAATTGCATTGGCTGGTAATAT
TACTGATATTAATGTATCAACTTTGCCGGATAGTCAAAAAATTATCAAAATCCGTTTTGACCGGGATGTGATTAGCCCGA
GCGGCTTTGTTACATCCACTCCGTCCCGAATTGCCTTGGATTTTACCAAAACAAATATTCAATTGGCTCAACCTGTATTG
GAATACGCGGATCCTTTGTTAAATCAAATTACTGCGGCTCAGAATAATGACCGATCTCGCATTGTTTTGGGATTGAATAA
AGCGGCGCAATATAATACCGAAGTCCATGGTAATGAAGTATGGGTATTCGTTACAGAATCATCTGATCGAACCACAACTT
CTACGGCTATTTCAAATAATGCAAATGCACGTCAGAAGGCAGGCTCGTCTGAAGCAGTTAAACAAACGGTAAATTCAGCT
AATATTGACTTCAGGAAGGGTGCACATAACTCAGGTATTGTGGAATTGAATGCGCCTGATTTCACAGGAAAACCTGAAGT
GAAGCAACAACGTGATCGGGTTATTATTACTCTGAAAAATTATCCGTTGCCGACACAAGCGCAACGCAGTTTGGATGTTG
CCGATTTCAGTACACCGGTTAAAAATATTACGTTAAAACGTATTGGAAATGATGCTCAACTCATTATTCGCAATAATGGT
AATTGGGATTTCAATACCAAAGCTGCAAATGGTCAATTTGCTTTTGAAGTAATGCCTAAGTCCGCAAGTACGGAATCAAG
TGGGCTGAATGCAAATCCAAACAAATCTTTCCGCGGCCGTAAAATTTCCCTGGACTTCCAAGATGTTGAAGTACGTACCA
TTTTGCAAATCCTTGCGAAGGAGTCAGGGGTTAATATCGTAGCCAGTGATTCTGTGAATGGCAAAATGACACTTTCTTTA
AAAGATGTGCCTTGGGATCAGGCTTTGGATTTGGTTATGCAGGCGCGTAATTTGGATATGCGCCGACAAGGCAATATTAT
CAATATCGCACCTCGTGATGAATTGTTGGCTAAAGATAAGGCTTTCTTGCAGGCGGAGAAAGAGATTGCCGAACTGGGGC
CTTTATATTCCCAAACTTTCCAATTGAAGTATAAGAATGTAGAAGAGTTCCGGAAAATTTTGCGTTTGGACGACAACAAT
AGTGGAAACTCCAATGGTAGAAATACCTTGTTGAGCGGCCGTGGCAGTGCGCTGATTGATCCTGCAACGAATACTTTGAT
TATTACGGATAATCGAAATGTTGTAGAAAAATTCCGTAAGTTGATTGAGGAATTGGATGTACCGACTCGTCAAGTTATGG
TTGAAGCCCGTATCGTTGAAGCAAAAGACGGTTTTTCGCGTGATTTGGGCGTGAAATTCGGTGTCGCAGGTTCCAAAGGA
CGCAACGCTTGGGGTAACTCTTGGAGTAATGCGCAAGATAACCATAATACTAATTCGGCTGTTTTGCGAGGTGATGCAAA
TTACCGTACATGGTCTTTGCAACCGAATATTAGCCTGCCTGCGGCAGCGGCGACTAATAATATTGCTTTGGTGCGAGCTT
TGTCATCGGGTGCATTAGGTTTGGAAATTTCTGCTTCGGAAGCTACCGGTAAAAGTAAGACCATTTCTAATCCGCGTGTC
CTGACACAAGACCGTAGAGAAGCTACTATTGAGTCTGGTACTGAAATTCCGTATCAGGAGGCATCTTCCAGTGGTGCAAC
CTCGGTATCGTTCAAAAAAGCCGTGCTGGGCTTAACGGTAACGCCGAATGTAACGCCTGATGGTCAAGTTATCATGACTG
TAAAAATTACCAAGGATACACCGCAAGACTGTACAGTGGACTCTTTGATTACGAAATGTATTCAGACTAAAAACTTGAAT
ACTCAGGCAATGGTGGAAGATGGTGGTACGTTGATCGTTGGTGGCATCTACGAAGAAGATAATAGCGATACGATTAACAA
AGTACCATTATTGGGCGATTTGCCAGTGGTGGGTAATTTGTTCAAGTCCCGTGCGAAAAAAGAAGAACGTCGTGAATTGC
TGGTTTTCATCACTCCGCGAATTATGGATGGTGTAGGCAGCAATCTCCGCTACTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(378-451)

Bacterial type II and III secretion system protein (Secretin)

(546-703)

AMIN domain (AMIN)

(41-120)

Secretin and TonB N terminus short domain (STN)

(304-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

62.027

100

0.646
  pilQ Neisseria meningitidis MC58

66.548

79.044

0.526
  pilQ Neisseria meningitidis M1080

66.905

78.622

0.526

Multiple sequence alignment