Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PFL_RS29585 Genome accession   NC_004129
Coordinates   6645826..6646860 (-) Length   344 a.a.
NCBI ID   WP_011064011.1    Uniprot ID   Q4K4D8
Organism   Pseudomonas protegens Pf-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6640826..6651860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFL_RS29555 (PFL_5831) ruvX 6640969..6641406 (+) 438 WP_011064005.1 Holliday junction resolvase RuvX -
  PFL_RS29560 (PFL_5832) pyrR 6641522..6642028 (+) 507 WP_011064006.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PFL_RS29565 (PFL_5833) - 6642054..6643058 (+) 1005 WP_011064007.1 aspartate carbamoyltransferase catalytic subunit -
  PFL_RS29570 (PFL_5834) - 6643055..6644326 (+) 1272 WP_011064008.1 dihydroorotase -
  PFL_RS29575 (PFL_5835) - 6644454..6644873 (-) 420 WP_011064009.1 NINE protein -
  PFL_RS29580 (PFL_5836) - 6645124..6645747 (+) 624 WP_011064010.1 C40 family peptidase -
  PFL_RS29585 (PFL_5837) pilT 6645826..6646860 (-) 1035 WP_011064011.1 type IV pilus twitching motility protein PilT Machinery gene
  PFL_RS29590 (PFL_5838) - 6647051..6647749 (+) 699 WP_011064012.1 YggS family pyridoxal phosphate-dependent enzyme -
  PFL_RS29595 (PFL_5839) proC 6647784..6648602 (+) 819 WP_011064013.1 pyrroline-5-carboxylate reductase -
  PFL_RS29600 (PFL_5840) - 6648613..6649203 (+) 591 WP_011064014.1 YggT family protein -
  PFL_RS29605 (PFL_5841) - 6649218..6649511 (+) 294 WP_011064015.1 DUF167 domain-containing protein -
  PFL_RS29610 (PFL_5842) metX 6649675..6650814 (+) 1140 WP_011064016.1 homoserine O-succinyltransferase MetX -
  PFL_RS29615 (PFL_5843) metW 6650822..6651442 (+) 621 WP_011064017.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37389.14 Da        Isoelectric Point: 7.0293

>NTDB_id=22313 PFL_RS29585 WP_011064011.1 6645826..6646860(-) (pilT) [Pseudomonas protegens Pf-5]
MDITELLRLSASRGASDLHLSAGLAPMLRIDGDIRPLPGPALEGPQVQALIHELMSEAQRQAFAACNDLDFAYELPGVAR
FRVNAFRHARGVGAVLRLIPARVQSLEELGLGEVFRQITEAPRGLVLVTGPTGSGKSTTLAAMIDHLNRNRQQHILTIED
PIEFVHPQKNCLVHQREVQRDTQSFATALRAALREDPDVILLGELRDLETIRLALTAAETGHLVLATLHTSSAAKTIDRL
VDVFPGEEKALVRSMLAESLQAVVAQVLVKKVGGGRVAAHEIMLGSPAIRNLIREDKVAQMYSAIQTGGAQGMRTLDMSL
KALLGQGLISRDQAREQARMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=22313 PFL_RS29585 WP_011064011.1 6645826..6646860(-) (pilT) [Pseudomonas protegens Pf-5]
ATGGACATCACCGAACTCTTGCGCTTGAGCGCTTCCCGGGGCGCTTCGGACCTGCATCTGTCCGCCGGGCTGGCGCCGAT
GTTGCGCATCGATGGCGATATTCGCCCGCTGCCGGGCCCGGCCCTGGAAGGGCCACAGGTGCAGGCGCTGATCCATGAAC
TGATGAGCGAAGCACAGCGCCAGGCGTTCGCCGCCTGCAATGACCTGGATTTCGCCTATGAACTGCCGGGTGTGGCGCGG
TTTCGGGTCAATGCCTTCCGGCACGCCCGTGGCGTCGGTGCGGTGCTGCGCCTGATTCCCGCGCGGGTACAGAGCCTGGA
GGAGCTGGGGCTGGGGGAAGTGTTTCGGCAGATTACCGAGGCGCCCCGCGGGCTGGTGCTGGTGACCGGGCCCACCGGCA
GCGGCAAGTCCACCACACTGGCGGCGATGATCGACCACCTGAACCGGAACCGCCAGCAGCACATCCTGACCATCGAAGAC
CCTATCGAATTCGTGCATCCGCAGAAGAACTGCCTGGTGCACCAGCGCGAAGTGCAGCGCGATACCCAGAGCTTCGCCAC
GGCCTTGCGTGCGGCGCTGCGGGAAGATCCCGACGTGATCCTGCTGGGCGAACTGCGGGACCTGGAAACCATCCGCCTGG
CCCTGACCGCCGCGGAAACCGGGCACCTGGTACTGGCCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGCTG
GTGGACGTGTTTCCGGGGGAGGAAAAGGCCCTGGTGCGCTCGATGCTGGCCGAGTCGCTGCAGGCAGTGGTCGCGCAAGT
GCTGGTGAAGAAGGTGGGCGGTGGCCGGGTAGCGGCGCACGAGATCATGCTGGGCAGCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACGGGCGGCGCCCAGGGCATGCGCACCCTGGACATGAGCCTC
AAGGCATTGCTCGGGCAGGGGTTGATCAGCCGCGATCAGGCGCGGGAGCAGGCGCGGATGCCGGAGAGCTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4K4D8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

70.93

100

0.709
  pilT Pseudomonas stutzeri DSM 10701

70.93

100

0.709
  pilT Acinetobacter baumannii D1279779

66.279

100

0.663
  pilT Acinetobacter baumannii strain A118

66.279

100

0.663
  pilT Acinetobacter nosocomialis M2

66.279

100

0.663
  pilT Acinetobacter baylyi ADP1

65.698

100

0.657
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.353

98.837

0.616
  pilT Vibrio cholerae strain A1552

62.353

98.837

0.616
  pilT Legionella pneumophila strain Lp02

61.834

98.256

0.608
  pilT Legionella pneumophila strain ERS1305867

61.834

98.256

0.608
  pilT Neisseria meningitidis 8013

58.261

100

0.584
  pilT Neisseria gonorrhoeae MS11

57.971

100

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.791

97.384

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

38.261

100

0.384

Multiple sequence alignment