Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   A6J57_RS02480 Genome accession   NZ_CP020405
Coordinates   521947..523281 (-) Length   444 a.a.
NCBI ID   WP_005757388.1    Uniprot ID   -
Organism   Pasteurella multocida strain FDAARGOS_218     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 516947..528281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J57_RS02455 (A6J57_02450) secM 518467..518778 (-) 312 WP_005723689.1 secA translation cis-regulator SecM -
  A6J57_RS02460 (A6J57_02455) - 518871..519176 (+) 306 WP_005723691.1 DUF721 domain-containing protein -
  A6J57_RS02465 (A6J57_02460) - 519223..520128 (-) 906 WP_005757382.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  A6J57_RS02470 (A6J57_02465) aroB 520132..521220 (-) 1089 WP_005757383.1 3-dehydroquinate synthase -
  A6J57_RS02475 (A6J57_02470) aroK 521237..521764 (-) 528 WP_005717623.1 shikimate kinase AroK -
  A6J57_RS02480 (A6J57_02475) comE 521947..523281 (-) 1335 WP_005757388.1 type IV pilus secretin PilQ Machinery gene
  A6J57_RS02485 (A6J57_02480) - 523291..523653 (-) 363 WP_005757389.1 pilus assembly protein PilP -
  A6J57_RS02490 (A6J57_02485) - 523656..524180 (-) 525 WP_005757390.1 hypothetical protein -
  A6J57_RS02495 (A6J57_02490) - 524180..524695 (-) 516 WP_005757392.1 hypothetical protein -
  A6J57_RS02500 (A6J57_02495) - 524735..525508 (-) 774 WP_005757393.1 pilus assembly protein PilM -
  A6J57_RS02505 (A6J57_02500) - 525644..528205 (+) 2562 WP_005757394.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49254.67 Da        Isoelectric Point: 8.0315

>NTDB_id=222264 A6J57_RS02480 WP_005757388.1 521947..523281(-) (comE) [Pasteurella multocida strain FDAARGOS_218]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVIAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=222264 A6J57_RS02480 WP_005757388.1 521947..523281(-) (comE) [Pasteurella multocida strain FDAARGOS_218]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTTGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCGGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGAATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGCGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCTGGGAGTTTAGCTGCAAACGGCTTTTCAAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCAGTTGGTTCCATCGCCCTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAACG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATTAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCATCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.037

97.297

0.604
  comE Haemophilus influenzae 86-028NP

61.343

97.297

0.597
  comE Glaesserella parasuis strain SC1401

48.961

97.523

0.477
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369

Multiple sequence alignment