Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   A6J55_RS04430 Genome accession   NZ_CP020403
Coordinates   947960..949294 (-) Length   444 a.a.
NCBI ID   WP_005754751.1    Uniprot ID   -
Organism   Pasteurella multocida strain FDAARGOS_216     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 942960..954294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J55_RS04405 (A6J55_04405) secM 944480..944791 (-) 312 WP_032851684.1 secA translation cis-regulator SecM -
  A6J55_RS04410 (A6J55_04410) - 944884..945189 (+) 306 WP_005723691.1 DUF721 domain-containing protein -
  A6J55_RS04415 (A6J55_04415) - 945236..946141 (-) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  A6J55_RS04420 (A6J55_04420) aroB 946145..947233 (-) 1089 WP_005754750.1 3-dehydroquinate synthase -
  A6J55_RS04425 (A6J55_04425) aroK 947250..947777 (-) 528 WP_005717623.1 shikimate kinase AroK -
  A6J55_RS04430 (A6J55_04430) comE 947960..949294 (-) 1335 WP_005754751.1 type IV pilus secretin PilQ Machinery gene
  A6J55_RS04435 (A6J55_04435) - 949304..949666 (-) 363 WP_005748555.1 pilus assembly protein PilP -
  A6J55_RS04440 (A6J55_04440) - 949669..950193 (-) 525 WP_005754754.1 hypothetical protein -
  A6J55_RS04445 (A6J55_04445) - 950193..950708 (-) 516 WP_005751904.1 hypothetical protein -
  A6J55_RS04450 (A6J55_04450) - 950748..951521 (-) 774 WP_005754755.1 pilus assembly protein PilM -
  A6J55_RS04455 (A6J55_04455) - 951657..954218 (+) 2562 WP_005754757.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49272.68 Da        Isoelectric Point: 8.0315

>NTDB_id=222244 A6J55_RS04430 WP_005754751.1 947960..949294(-) (comE) [Pasteurella multocida strain FDAARGOS_216]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DELQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
TPVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNSILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=222244 A6J55_RS04430 WP_005754751.1 947960..949294(-) (comE) [Pasteurella multocida strain FDAARGOS_216]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCACTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACTGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
ACACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACAG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACTCGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCAAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.037

97.297

0.604
  comE Haemophilus influenzae 86-028NP

61.343

97.297

0.597
  comE Glaesserella parasuis strain SC1401

48.961

97.523

0.477
  pilQ Vibrio campbellii strain DS40M4

39.286

94.595

0.372
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.32

92.793

0.365
  pilQ Vibrio cholerae strain A1552

39.32

92.793

0.365

Multiple sequence alignment