Detailed information NTG0220616

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: CXX09_RS16330
• Genome accession: NZ_CP025865
• Coordinates: 3344697..3345677 (+)
• Length: 326 a.a.
• NCBI ID: WP_001055620.1
• Uniprot ID: -
• Organism: Escherichia coli strain 504211
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3339697..3350677

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CXX09_RS16300	yggM	3340125..3341132 (+)	1008	WP_000745217.1	DUF1202 family protein	-
CXX09_RS16305	hemW	3341374..3342510 (-)	1137	WP_000239922.1	radical SAM family heme chaperone HemW	-
CXX09_RS16310	rdgB	3342503..3343096 (-)	594	WP_001174743.1	XTP/dITP diphosphatase	-
CXX09_RS16315	yggU	3343104..3343394 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
CXX09_RS16320	yggT	3343391..3343957 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
CXX09_RS16325	yggS	3343975..3344679 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
CXX09_RS16330	pilT	3344697..3345677 (+)	981	WP_001055620.1	type IV pilus twitching motility protein PilT	Machinery gene
CXX09_RS16335	ruvX	3345853..3346269 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
CXX09_RS16340	yqgE	3346269..3346832 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
CXX09_RS16345	gshB	3346941..3347891 (-)	951	WP_000593284.1	glutathione synthase	-
CXX09_RS16350	rsmE	3347904..3348635 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
CXX09_RS16355	endA	3348715..3349422 (-)	708	WP_000286500.1	deoxyribonuclease I	-
CXX09_RS16360	yggI	3349517..3350014 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35903.08 Da
• Isoelectric Point: 5.5860

>NTDB_id=220616 CXX09_RS16330 WP_001055620.1 3344697..3345677(+) (pilT) [Escherichia coli strain 504211]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=220616 CXX09_RS16330 WP_001055620.1 3344697..3345677(+) (pilT) [Escherichia coli strain 504211]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.346	99.387	0.371
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365