Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   B4W66_RS00595 Genome accession   NZ_CP020027
Coordinates   97138..98172 (+) Length   344 a.a.
NCBI ID   WP_228631569.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain STAB090229     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 92138..103172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4W66_RS00585 (B4W66_00585) - 95806..96171 (+) 366 WP_002986560.1 DUF1033 family protein -
  B4W66_RS00590 (B4W66_00590) comYA 96264..97202 (+) 939 WP_002986557.1 competence type IV pilus ATPase ComGA Machinery gene
  B4W66_RS00595 (B4W66_00595) comYB 97138..98172 (+) 1035 WP_228631569.1 competence type IV pilus assembly protein ComGB Machinery gene
  B4W66_RS00600 (B4W66_00600) comYC 98174..98500 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  B4W66_RS00605 (B4W66_00605) comGD 98475..98903 (+) 429 WP_076639399.1 competence type IV pilus minor pilin ComGD -
  B4W66_RS00610 (B4W66_00610) comGE 98860..99144 (+) 285 WP_307777288.1 competence type IV pilus minor pilin ComGE -
  B4W66_RS00615 (B4W66_00615) comYF 99137..99571 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  B4W66_RS00620 (B4W66_00620) comGG 99555..99881 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  B4W66_RS00625 (B4W66_00625) comYH 99979..100932 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  B4W66_RS00630 (B4W66_00630) - 100991..102187 (+) 1197 WP_020904834.1 acetate kinase -
  B4W66_RS00635 (B4W66_00635) - 102374..102682 (+) 309 Protein_90 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39648.10 Da        Isoelectric Point: 9.7085

>NTDB_id=220404 B4W66_RS00595 WP_228631569.1 97138..98172(+) (comYB) [Streptococcus pyogenes strain STAB090229]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTAEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=220404 B4W66_RS00595 WP_228631569.1 97138..98172(+) (comYB) [Streptococcus pyogenes strain STAB090229]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGCTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCCGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment