Detailed information NTG0218675

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: XJ28_RS26935
• Genome accession: NZ_CP019871
• Coordinates: 5603494..5604558 (-)
• Length: 354 a.a.
• NCBI ID: WP_005765505.1
• Uniprot ID: A0A3M3ZC03
• Organism: Pseudomonas syringae pv. tomato strain B13-200
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5598494..5609558

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XJ28_RS26910 (XJ28_26505)	aroK	5599048..5599566 (-)	519	WP_007245596.1	shikimate kinase AroK	-
XJ28_RS26915 (XJ28_26510)	pilQ	5599571..5601676 (-)	2106	WP_005765499.1	type IV pilus secretin PilQ	Machinery gene
XJ28_RS26920 (XJ28_26515)	pilP	5601724..5602251 (-)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
XJ28_RS26925 (XJ28_26520)	pilO	5602248..5602871 (-)	624	WP_005765501.1	type 4a pilus biogenesis protein PilO	-
XJ28_RS26930 (XJ28_26525)	-	5602868..5603494 (-)	627	WP_005765503.1	PilN domain-containing protein	-
XJ28_RS26935 (XJ28_26530)	pilM	5603494..5604558 (-)	1065	WP_005765505.1	pilus assembly protein PilM	Machinery gene
XJ28_RS26940 (XJ28_26535)	-	5604768..5607209 (+)	2442	WP_371245724.1	penicillin-binding protein 1A	-
XJ28_RS26945 (XJ28_26540)	-	5607411..5608679 (-)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38117.65 Da
• Isoelectric Point: 4.7008

>NTDB_id=218675 XJ28_RS26935 WP_005765505.1 5603494..5604558(-) (pilM) [Pseudomonas syringae pv. tomato strain B13-200]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLIKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=218675 XJ28_RS26935 WP_005765505.1 5603494..5604558(-) (pilM) [Pseudomonas syringae pv. tomato strain B13-200]
GTGTTCGAACTCTTTGGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTACTGATCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCGGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
CAAGCTTGAAGCTGATCAGTACATCCCTTACCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAAGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGTCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACCGTTGCCGTGGTTGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCATGGTC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGCCAGCTGACTGACGAAATTCAGCGCCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAGGTGCTGGACCCCTTCAAGGACGC
GCTGGTGCAACAGGTTTCCCGATCCCTGCAATTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCGTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACACCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGTTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCACCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A3M3ZC03

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469