Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B1777_RS00270 Genome accession   NZ_CP019867
Coordinates   54118..55512 (+) Length   464 a.a.
NCBI ID   WP_012983997.1    Uniprot ID   A0A1S6SF54
Organism   Dehalococcoides mccartyi strain KBDCA1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 49118..60512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1777_RS00265 (B1777_00265) - 51254..53728 (+) 2475 WP_077997810.1 ATP-dependent Clp protease ATP-binding subunit -
  B1777_RS00270 (B1777_00270) radA 54118..55512 (+) 1395 WP_012983997.1 DNA repair protein RadA Machinery gene
  B1777_RS00275 (B1777_00275) - 55499..56182 (+) 684 WP_012983998.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  B1777_RS00280 (B1777_00280) ispF 56188..56661 (+) 474 WP_011308693.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  B1777_RS00285 (B1777_00285) cysS 56665..58038 (+) 1374 WP_058316829.1 cysteine--tRNA ligase -
  B1777_RS00295 (B1777_00295) - 58257..59255 (+) 999 WP_011928581.1 tyrosine-type recombinase/integrase -
  B1777_RS00300 (B1777_00300) - 59282..60148 (-) 867 WP_011928582.1 Bro-N domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 49429.78 Da        Isoelectric Point: 6.6399

>NTDB_id=218600 B1777_RS00270 WP_012983997.1 54118..55512(+) (radA) [Dehalococcoides mccartyi strain KBDCA1]
MDKSRNVYICSNCGHESLKWLGRCPGCQEWNTLEETTIAAPLGRKNAPARVISPAAELSSLNASDTTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLCPSLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLIRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTIMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDIGVCPETIALGEIGLSGELR
TIPHLERRLSEASRLGFTRALVPAGANCQNINIHGIQIIAVSTVKEAIKLALTGVKTETEDVFE

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=218600 B1777_RS00270 WP_012983997.1 54118..55512(+) (radA) [Dehalococcoides mccartyi strain KBDCA1]
ATGGATAAAAGCCGTAATGTTTATATCTGTTCCAACTGCGGACATGAGAGCCTGAAGTGGCTGGGGCGTTGCCCCGGCTG
TCAGGAATGGAATACTCTTGAAGAAACAACAATTGCTGCACCTCTCGGGCGTAAAAATGCACCTGCCCGGGTTATCAGCC
CGGCAGCCGAACTTTCCAGTCTGAATGCATCAGATACTACCCGCCGGAGTCTATCCATTTCAGAGTTCAACCGGGTTTTG
GGCGGTGGAATAGTGCCGGGTTCACTGATGCTTTTAGGTGGTGAACCGGGTATCGGCAAATCCACGCTCCTTCTTCAGGT
GGCTGCCTCGGTAGCCCAGAGCGGCGGCAAAGTGGTATATGTTTCCGGGGAAGAGAACCCCGCCCAGATAAAAATGCGCG
CTCAGCGTCTGGGTATCAGCGGCGAGGGGCTTTTCCTTATGGCTGAGACAGACCTTAATGCCATTCTAGCCCAGCTTTCA
GTCCTCTGCCCGTCATTGGTTGTTATAGACTCCATCCAGACTGTATTCCTGCCTGAGCTTGAGGCCGCACCCGGTGCTAT
AAATCAGGTGCGTGAATCAGCCCTCCGCCTGATGCAGTGGGCCAAGAGCAGCGGTGCCAGCGTATTTATTACCGCCCATG
TTACCAAAGAGGGCAACATCGCCGGGCCGCGCATACTGGAACATATAGTAGATGTGGTCATGTACTTTGAGGGCGAGTCC
CAGAGTGCTTACCGTTTGATACGTTCGGTTAAAAACCGTTTCGGTTCTACCAACGAAGTAGGCATATTTGAAATGAAAAG
TGAAGGATTGGTGGAAGTAGCCAATCCCTCGCAGATATTTTTATCTAACCGTCAGGCAAACACTGTTGGTTCAGCTGTAA
CAGCAGTGCTGGAGGGTAGCCGCCCTTTACTGGTAGAGGTGCAGGCACTCACCAATACCACCAGTTTCGGCCAGCCGCGC
CGCACGGCCAACGGGGTAGATTTTAACCGTACTATTATGATAGCCGCTGTTCTTTCCAAACGCCTTTCCATGCGGCTGGG
TACCCAGGACATAATAGTAAATGCCACCGGCGGTATTCGTCTGGACGAGCCGGCCGCAGATTTGGCTATTGCTTTGGCCA
TTGCCTCCAGTTACCGTGATATCGGGGTCTGCCCGGAAACCATAGCACTGGGTGAGATTGGCCTTTCAGGGGAACTGAGG
ACAATCCCTCATTTGGAAAGACGTCTTTCCGAGGCCAGCCGTTTGGGTTTTACGAGGGCTTTGGTACCTGCCGGTGCTAA
TTGCCAGAATATAAATATACATGGTATCCAGATTATTGCGGTTTCAACTGTCAAAGAGGCTATTAAACTGGCGCTTACCG
GGGTAAAAACGGAGACCGAAGATGTTTTTGAATGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(335-424)

AAA domain (AAA_25)

(66-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S6SF54

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.218

98.707

0.496
  radA Streptococcus mitis SK321

48.578

98.491

0.478
  radA Streptococcus mitis NCTC 12261

48.359

98.491

0.476
  radA Streptococcus pneumoniae TIGR4

48.14

98.491

0.474
  radA Streptococcus pneumoniae R6

48.14

98.491

0.474
  radA Streptococcus pneumoniae Rx1

48.14

98.491

0.474
  radA Streptococcus pneumoniae D39

48.14

98.491

0.474

Multiple sequence alignment