Detailed information NTG0218163

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: B1R35_RS27870
• Genome accession: NZ_CP019730
• Coordinates: 5832209..5833273 (-)
• Length: 354 a.a.
• NCBI ID: WP_003378852.1
• Uniprot ID: A0A2G9KZJ9
• Organism: Pseudomonas syringae pv. actinidiae strain CRAFRU 12.29
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5827209..5838273

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B1R35_RS27845 (B1R35_28120)	aroK	5827763..5828281 (-)	519	WP_003378847.1	shikimate kinase AroK	-
B1R35_RS27850 (B1R35_28125)	pilQ	5828286..5830391 (-)	2106	WP_017682642.1	type IV pilus secretin PilQ	Machinery gene
B1R35_RS27855 (B1R35_28130)	pilP	5830439..5830966 (-)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
B1R35_RS27860 (B1R35_28135)	pilO	5830963..5831586 (-)	624	WP_003378850.1	type 4a pilus biogenesis protein PilO	-
B1R35_RS27865 (B1R35_28140)	-	5831583..5832209 (-)	627	WP_003378851.1	PilN domain-containing protein	-
B1R35_RS27870 (B1R35_28145)	pilM	5832209..5833273 (-)	1065	WP_003378852.1	pilus assembly protein PilM	Machinery gene
B1R35_RS27875 (B1R35_28150)	-	5833483..5835924 (+)	2442	WP_370445385.1	penicillin-binding protein 1A	-
B1R35_RS27880 (B1R35_28155)	-	5836250..5837518 (-)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38103.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=218163 B1R35_RS27870 WP_003378852.1 5832209..5833273(-) (pilM) [Pseudomonas syringae pv. actinidiae strain CRAFRU 12.29]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=218163 B1R35_RS27870 WP_003378852.1 5832209..5833273(-) (pilM) [Pseudomonas syringae pv. actinidiae strain CRAFRU 12.29]
GTGTTCGAACTCTTTGGCAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCTCGCGTACTGGTCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
TAAGCTTGAAGCTGATCAGTACATCCCTTATCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGTTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTC
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGGCTTCTGGCTGCGCAACTGGG
CAACGGTCATGACGAACTGACCGTTGCCGTGGTAGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCACGGCC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTGACTGACGAAATTCAGCGTCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAAGTGCTGGACCCCTTCAAGGACGC
GCTCGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACGCCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGCTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2G9KZJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469