Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   Mag101_RS01320 Genome accession   NZ_CP019650
Coordinates   313452..314528 (+) Length   358 a.a.
NCBI ID   WP_077399711.1    Uniprot ID   A0A1Q2M2I0
Organism   Microbulbifer agarilyticus strain GP101     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 308452..319528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Mag101_RS01310 (Mag101_01345) - 309995..310486 (+) 492 WP_077399705.1 chemotaxis protein CheW -
  Mag101_RS01315 (Mag101_01350) - 310606..313296 (-) 2691 WP_077399708.1 penicillin-binding protein 1A -
  Mag101_RS01320 (Mag101_01355) pilM 313452..314528 (+) 1077 WP_077399711.1 pilus assembly protein PilM Machinery gene
  Mag101_RS01325 (Mag101_01360) - 314532..315116 (+) 585 WP_077399714.1 PilN domain-containing protein -
  Mag101_RS01330 (Mag101_01365) - 315119..315739 (+) 621 WP_077399718.1 type 4a pilus biogenesis protein PilO -
  Mag101_RS01335 (Mag101_01370) - 315736..316263 (+) 528 WP_077399721.1 pilus assembly protein PilP -
  Mag101_RS01340 (Mag101_01375) pilQ 316265..318490 (+) 2226 WP_077399724.1 type IV pilus secretin PilQ family protein -
  Mag101_RS01345 (Mag101_01380) - 318520..319011 (+) 492 WP_232325088.1 shikimate kinase -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38915.23 Da        Isoelectric Point: 4.3351

>NTDB_id=217561 Mag101_RS01320 WP_077399711.1 313452..314528(+) (pilM) [Microbulbifer agarilyticus strain GP101]
MGMLPFLNKGPKAMLGLDISSTSVKLLELSRSGDKYRVESYAAEPLPPNAVVDKNINDVGAIAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLDMPADLSDDALEAQIALEADQYIPYPLDEVNLDFEVQGPSEKGEGQVEVLLAACRSENVEQRISAL
GEAELQPGVVDVEAYAIERAYTLLDNQFPAQEQLVVAVIDIGATMSALSVMVDGRTVYTREQLFGGRQLTDEIQRRYGLS
AEEATLAKRQGGSGLPDDYYPEVLEPFRDAVVQQVMRSLQFFYSSTSYSDVDYILLGGGVAAMDGLAELVGEKLNKPTLV
ANPFRDMSVASRVNRQALVSEAPSLMIAAGLAMREREF

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=217561 Mag101_RS01320 WP_077399711.1 313452..314528(+) (pilM) [Microbulbifer agarilyticus strain GP101]
ATGGGGATGCTCCCTTTTCTCAATAAGGGCCCGAAAGCCATGCTTGGGCTCGATATTAGCTCCACCTCGGTCAAGTTGCT
CGAACTGAGTCGCAGTGGCGACAAATATCGGGTGGAAAGCTATGCAGCCGAGCCTCTGCCCCCGAATGCGGTAGTGGATA
AGAACATCAATGATGTCGGTGCCATTGCCGAGGCTATCCGCAAGGTCGTGCGCCGGTCCAAGACCCGCCTGCGTCAGGCG
GCTGTGGCTGTTTCCGGCTCTGCGGTCATTACCAAGACCCTGGATATGCCCGCCGACCTCTCCGACGATGCGCTGGAAGC
CCAGATTGCGCTGGAAGCGGATCAGTACATTCCCTACCCCCTGGACGAGGTAAATCTCGACTTCGAGGTGCAGGGACCCT
CCGAAAAAGGAGAGGGCCAGGTTGAGGTGCTGCTGGCCGCGTGTCGCAGCGAGAACGTGGAGCAGCGGATTTCAGCGTTG
GGCGAGGCCGAGTTGCAGCCGGGCGTGGTGGATGTAGAAGCCTACGCCATCGAGCGCGCTTATACCCTGTTGGACAATCA
GTTTCCGGCCCAGGAGCAGCTGGTGGTCGCGGTAATTGATATTGGTGCGACTATGAGTGCTCTGTCAGTCATGGTTGACG
GTCGCACTGTATACACCCGTGAACAGTTGTTTGGTGGTCGCCAGTTGACCGATGAAATCCAGCGCCGTTACGGGCTGTCA
GCAGAAGAGGCCACTCTGGCCAAGCGTCAGGGCGGCAGCGGCTTGCCTGACGACTACTACCCGGAAGTGCTTGAGCCCTT
CCGCGATGCGGTGGTGCAGCAAGTGATGCGCTCCCTGCAGTTCTTCTATTCCTCAACTTCGTACAGCGACGTGGACTACA
TCCTGCTGGGTGGCGGTGTTGCCGCCATGGACGGGCTCGCCGAACTGGTGGGTGAAAAACTTAACAAGCCTACGCTCGTG
GCCAACCCGTTCCGGGATATGAGTGTCGCGTCCCGGGTGAACCGCCAGGCTCTGGTGAGTGAGGCACCGTCCCTGATGAT
CGCTGCAGGCCTCGCCATGCGCGAAAGGGAGTTCTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q2M2I0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.705

98.324

0.508
  comM Acinetobacter nosocomialis M2

51.705

98.324

0.508
  comM Acinetobacter baylyi ADP1

47.727

98.324

0.469
  pilM Legionella pneumophila strain ERS1305867

44.944

99.441

0.447

Multiple sequence alignment