Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BZ167_RS17495 Genome accession   NZ_CP019626
Coordinates   3539940..3541010 (+) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus sp. 275     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3534940..3546010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BZ167_RS17465 (BZ167_17540) - 3535413..3535658 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  BZ167_RS17470 (BZ167_17545) - 3535895..3536275 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BZ167_RS17480 (BZ167_17555) - 3536482..3537312 (+) 831 WP_015417821.1 STAS domain-containing protein -
  BZ167_RS17485 (BZ167_17560) - 3537350..3538651 (-) 1302 WP_012117986.1 hemolysin family protein -
  BZ167_RS17490 (BZ167_17565) - 3538797..3539747 (+) 951 WP_015417820.1 magnesium transporter CorA family protein -
  BZ167_RS17495 (BZ167_17570) comGA 3539940..3541010 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  BZ167_RS17500 (BZ167_17575) comGB 3540997..3542034 (+) 1038 WP_015417819.1 competence type IV pilus assembly protein ComGB Machinery gene
  BZ167_RS17505 (BZ167_17580) comGC 3542081..3542347 (+) 267 WP_050515801.1 competence type IV pilus major pilin ComGC Machinery gene
  BZ167_RS17510 (BZ167_17585) comGD 3542337..3542774 (+) 438 WP_015417817.1 competence type IV pilus minor pilin ComGD Machinery gene
  BZ167_RS17515 (BZ167_17590) comGE 3542758..3543072 (+) 315 WP_031378943.1 competence type IV pilus minor pilin ComGE -
  BZ167_RS17520 (BZ167_17595) comGF 3542981..3543481 (+) 501 WP_258548905.1 competence type IV pilus minor pilin ComGF -
  BZ167_RS17525 (BZ167_17600) comGG 3543482..3543859 (+) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  BZ167_RS17530 (BZ167_17605) - 3543916..3544095 (+) 180 WP_003153093.1 YqzE family protein -
  BZ167_RS17535 (BZ167_17610) - 3544135..3544464 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  BZ167_RS17540 (BZ167_17615) tapA 3544723..3545394 (+) 672 WP_015417813.1 amyloid fiber anchoring/assembly protein TapA -
  BZ167_RS17545 (BZ167_17620) - 3545366..3545950 (+) 585 WP_015240205.1 signal peptidase I -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=217383 BZ167_RS17495 WP_003153083.1 3539940..3541010(+) (comGA) [Bacillus sp. 275]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=217383 BZ167_RS17495 WP_003153083.1 3539940..3541010(+) (comGA) [Bacillus sp. 275]
GTGAAAAATATTGAAACATATAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATTTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment