Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CWI24_RS28865 Genome accession   NZ_CP025050
Coordinates   5984148..5984771 (-) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain PB368     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5979148..5989771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI24_RS28845 (CWI24_28845) aroB 5979755..5980861 (-) 1107 WP_009314395.1 3-dehydroquinate synthase -
  CWI24_RS28850 (CWI24_28850) aroK 5980911..5981429 (-) 519 WP_003095833.1 shikimate kinase AroK -
  CWI24_RS28855 (CWI24_28855) pilQ 5981441..5983573 (-) 2133 WP_003141780.1 type 4a pilus secretin PilQ Machinery gene
  CWI24_RS28860 (CWI24_28860) comL/pilP 5983627..5984151 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  CWI24_RS28865 (CWI24_28865) pilO 5984148..5984771 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  CWI24_RS28870 (CWI24_28870) pilN 5984768..5985364 (-) 597 WP_043083103.1 type 4a pilus biogenesis protein PilN -
  CWI24_RS28875 (CWI24_28875) pilM 5985364..5986428 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  CWI24_RS28880 (CWI24_28880) - 5986613..5989084 (+) 2472 WP_043088268.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=216827 CWI24_RS28865 WP_003116135.1 5984148..5984771(-) (pilO) [Pseudomonas aeruginosa strain PB368]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=216827 CWI24_RS28865 WP_003116135.1 5984148..5984771(-) (pilO) [Pseudomonas aeruginosa strain PB368]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396