Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   BWR56_RS00175 Genome accession   NZ_CP019562
Coordinates   34035..35333 (+) Length   432 a.a.
NCBI ID   WP_061421558.1    Uniprot ID   A0A1L8Q4A3
Organism   Streptococcus oralis strain S.MIT/ORALIS-351     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 29035..40333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWR56_RS00150 (BWR56_0030) rpsB 29859..30638 (+) 780 WP_000268475.1 30S ribosomal protein S2 -
  BWR56_RS00155 (BWR56_0031) tsf 30717..31757 (+) 1041 WP_000808059.1 translation elongation factor Ts -
  BWR56_RS00160 (BWR56_0032) cysK 31861..32787 (-) 927 WP_061421561.1 cysteine synthase A -
  BWR56_RS00165 (BWR56_0033) - 32884..33327 (-) 444 WP_061421560.1 PH domain-containing protein -
  BWR56_RS00170 (BWR56_0034) - 33343..33978 (-) 636 WP_061421559.1 YigZ family protein -
  BWR56_RS00175 (BWR56_0035) comFA/cflA 34035..35333 (+) 1299 WP_061421558.1 DEAD/DEAH box helicase Machinery gene
  BWR56_RS00180 (BWR56_0036) comFC/cflB 35330..35992 (+) 663 WP_076984216.1 ComF family protein Machinery gene
  BWR56_RS00185 (BWR56_0037) hpf 36072..36620 (+) 549 WP_000599103.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BWR56_RS00190 (BWR56_0038) - 36702..37880 (+) 1179 WP_076984217.1 acetyl-CoA C-acetyltransferase -
  BWR56_RS00195 (BWR56_0039) - 38038..40011 (+) 1974 WP_076984218.1 DHH family phosphoesterase -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49510.41 Da        Isoelectric Point: 8.1535

>NTDB_id=216290 BWR56_RS00175 WP_061421558.1 34035..35333(+) (comFA/cflA) [Streptococcus oralis strain S.MIT/ORALIS-351]
MKVNPNYLGRLFTEKELTEEERQVAVRLPAMRKEKGKLFCQRCNSSILEEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPKQDVLKWCGQLTYFQEKVSEGLIRAVEKKEPTLVHAVTGAGKTEMIYQVVAKVIDDGGAVCLASPRIDVCLELYKR
LQNDFACEIALLHGESEPYFRTPLVVATTHQLLKFYHAFDLLIVDEVDAFPYVDNTMLYYAVKNSVKEDGLRIFLTATST
DELDRKVRTGELKRLSLPRRFHGNPLIIPKPVWLSDFNRCLEKSQLSTKLKTYIEKQRRTGYPLLIFASEIKKGEGLKEI
LNVHFPNESIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGELLFFHDGLNASIKKAIKEIKQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=216290 BWR56_RS00175 WP_061421558.1 34035..35333(+) (comFA/cflA) [Streptococcus oralis strain S.MIT/ORALIS-351]
ATGAAAGTAAATCCAAATTACCTCGGTCGCTTGTTTACTGAGAAAGAATTAACGGAAGAAGAACGACAGGTAGCAGTGAG
ACTGCCAGCAATGAGAAAAGAGAAGGGGAAACTGTTTTGTCAACGTTGTAATAGTTCGATTCTAGAAGAATGGTATTTGC
CTATTGGCGCATACTATTGTAGGGAGTGCTTACTGATGAAGCGAGTCAGGAGTGATCAAGCCTTATACTATTTTCCGCAA
GAAGATTTTCCTAAGCAAGATGTCCTCAAATGGTGTGGTCAGTTAACCTATTTTCAGGAAAAGGTATCAGAGGGGTTGAT
TCGAGCAGTTGAAAAGAAAGAACCGACTTTAGTTCACGCTGTCACAGGAGCTGGAAAGACAGAGATGATTTATCAAGTTG
TTGCCAAAGTGATTGATGATGGTGGTGCAGTTTGTTTGGCAAGTCCTCGAATTGATGTGTGTTTGGAACTGTATAAGCGA
CTGCAGAATGACTTTGCTTGTGAGATAGCACTACTCCATGGCGAATCAGAGCCCTATTTTCGAACACCCTTAGTAGTTGC
AACAACTCATCAGTTATTAAAATTTTATCATGCTTTTGATTTGTTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTCG
ACAACACTATGCTTTACTATGCTGTAAAGAATAGTGTAAAGGAGGATGGATTGAGGATATTCCTTACAGCAACTTCTACA
GATGAGTTAGATAGGAAGGTTCGCACAGGAGAATTAAAACGATTAAGCTTGCCGAGACGGTTTCATGGAAATCCTTTGAT
TATTCCTAAACCAGTCTGGTTATCGGATTTTAATCGCTGTTTAGAGAAAAGCCAGTTATCAACCAAGTTAAAGACCTATA
TTGAGAAACAGAGAAGAACAGGTTATCCATTGCTGATTTTTGCATCAGAGATTAAGAAAGGTGAGGGACTAAAAGAAATA
CTAAATGTGCATTTCCCGAATGAGAGCATTGGCTTTGTGTCTTCTGTGACAGAAGACCGATTAGAGCAGGTGCAAGCTTT
TCGAGATGGAGAACTAACAATACTGATCAGTACGACAATCTTGGAACGCGGGGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAAGCTAATCATCGTCTCTTTACCAAGTCTAGTTTAATTCAGATTGGAGGGCGAGTTGGACGCAGTATGGATAGA
CCAACTGGTGAGTTGCTCTTCTTTCATGATGGATTAAATGCTTCCATCAAAAAGGCAATCAAGGAAATCAAGCAGATGAA
TAAGGAGGCAGGGTTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L8Q4A3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

90.509

100

0.905
  comFA/cflA Streptococcus mitis SK321

90.046

100

0.9
  comFA/cflA Streptococcus pneumoniae Rx1

89.352

100

0.894
  comFA/cflA Streptococcus pneumoniae D39

89.352

100

0.894
  comFA/cflA Streptococcus pneumoniae R6

89.352

100

0.894
  comFA/cflA Streptococcus pneumoniae TIGR4

89.12

100

0.891
  comFA Lactococcus lactis subsp. cremoris KW2

50.856

94.676

0.481
  comFA Latilactobacillus sakei subsp. sakei 23K

38.952

100

0.396

Multiple sequence alignment