Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BST96_RS05655 Genome accession   NZ_CP019343
Coordinates   1259517..1260698 (-) Length   393 a.a.
NCBI ID   WP_085757764.1    Uniprot ID   A0A1X9N6B9
Organism   Oceanicoccus sagamiensis strain NBRC 107125     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1254517..1265698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BST96_RS05625 (BST96_05635) - 1254928..1255389 (-) 462 WP_085757759.1 PaaI family thioesterase -
  BST96_RS05630 (BST96_05640) - 1255508..1255927 (+) 420 WP_240554900.1 hypothetical protein -
  BST96_RS20925 - 1255906..1256139 (+) 234 WP_240554901.1 hypothetical protein -
  BST96_RS05635 (BST96_05645) - 1256136..1256495 (+) 360 WP_085757760.1 glutaredoxin family protein -
  BST96_RS05640 (BST96_05650) - 1256522..1256959 (+) 438 WP_085757761.1 MAPEG family protein -
  BST96_RS05645 (BST96_05655) - 1257331..1259130 (+) 1800 WP_085757762.1 DUF1631 family protein -
  BST96_RS05650 (BST96_05660) - 1259133..1259480 (+) 348 WP_085757763.1 PilZ domain-containing protein -
  BST96_RS05655 (BST96_05665) pilU 1259517..1260698 (-) 1182 WP_085757764.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BST96_RS05660 (BST96_05670) - 1260790..1261422 (-) 633 WP_085757765.1 DUF924 family protein -
  BST96_RS05665 (BST96_05675) gshA 1261571..1263154 (+) 1584 WP_085757766.1 glutamate--cysteine ligase -
  BST96_RS05670 (BST96_05680) - 1263191..1263775 (+) 585 WP_085757767.1 ACP phosphodiesterase -
  BST96_RS05675 (BST96_05685) - 1263780..1264070 (-) 291 WP_240554902.1 flagellar basal body-associated FliL family protein -
  BST96_RS20365 - 1264082..1264219 (-) 138 WP_157117869.1 hypothetical protein -
  BST96_RS05680 (BST96_05690) - 1264356..1264838 (+) 483 WP_085757769.1 disulfide bond formation protein B -
  BST96_RS05685 (BST96_05695) - 1264841..1265197 (+) 357 WP_085757770.1 CidA/LrgA family protein -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 43679.75 Da        Isoelectric Point: 5.0703

>NTDB_id=214951 BST96_RS05655 WP_085757764.1 1259517..1260698(-) (pilU) [Oceanicoccus sagamiensis strain NBRC 107125]
MEFADYLKILASKDGSDLYLSTGAPPCAKFEGVLKPLEKVAMQPGRIKDIAYELMDKRQQEEFEEELEMNLATSIPSVGR
FRVNIFKQRNEISIVARNIKTEIPNFDDLKLPEILKDVIVRKRGLVLFVGGTGSGKSTSLAALIDHRNSTSSGHIITIED
PVEFVHRHKKSVVNQREVGVDTRNFSNALKNTLRQAPDVILIGEVRDRDTMEHCLAFAETGHLAISTLHANNANQALDRI
INFFPEERRAQLLMDLSSNIQAFVSQRLIPTVDGKRVAAVEILLGTATIKEKILRGELEDIKEIMAKSENLGMQTFDTAL
FKLYKEGRITLEEAIKNADSANNLRLKIKLSDEGGVDSSASSATDSGGGFNLSLEEIEEDAEDEVPDNPFLKK

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=214951 BST96_RS05655 WP_085757764.1 1259517..1260698(-) (pilU) [Oceanicoccus sagamiensis strain NBRC 107125]
ATGGAATTTGCAGATTACTTAAAAATACTGGCTTCTAAAGATGGCTCCGACTTATACCTGAGTACCGGGGCACCTCCCTG
CGCCAAATTTGAGGGGGTGTTAAAACCGCTGGAAAAGGTCGCTATGCAGCCCGGGCGGATCAAGGATATTGCCTATGAGT
TAATGGACAAAAGACAGCAGGAAGAGTTTGAAGAAGAGTTGGAAATGAATTTGGCGACGTCTATCCCCAGTGTCGGCCGC
TTCCGGGTCAATATTTTTAAGCAGCGCAATGAAATTTCTATTGTGGCCCGCAATATCAAAACCGAAATTCCCAACTTTGA
TGATCTAAAGCTGCCAGAGATTTTAAAAGATGTCATCGTACGCAAGCGCGGTCTGGTTTTGTTTGTTGGTGGCACAGGTT
CGGGTAAGTCCACCTCATTGGCCGCGTTAATTGATCATCGCAATAGCACCAGTTCCGGTCATATTATTACGATTGAAGAC
CCGGTTGAATTTGTTCATCGCCATAAAAAATCCGTGGTCAATCAGCGCGAAGTGGGTGTTGATACACGTAATTTTAGTAA
TGCCTTAAAAAATACCTTGCGACAGGCGCCGGATGTTATTTTGATTGGTGAGGTCCGTGACCGCGATACCATGGAACATT
GCCTGGCGTTTGCCGAAACTGGCCACTTAGCGATTTCAACCTTGCATGCGAATAATGCTAATCAGGCTCTGGATAGAATT
ATTAATTTTTTCCCGGAAGAGCGTCGCGCACAATTATTAATGGATTTATCCTCCAATATACAGGCCTTTGTTTCGCAGCG
CTTAATCCCAACGGTGGATGGTAAGCGGGTGGCGGCGGTTGAGATTTTATTGGGTACGGCCACCATCAAAGAAAAAATTC
TACGGGGCGAGCTGGAAGATATTAAAGAGATTATGGCCAAGTCGGAAAACCTGGGCATGCAAACGTTCGATACGGCCCTA
TTTAAGCTTTATAAGGAAGGGCGAATCACGTTGGAAGAGGCGATTAAAAATGCCGATTCCGCCAATAATTTAAGATTGAA
AATAAAATTAAGTGATGAAGGCGGTGTTGATTCTTCGGCGAGTTCGGCGACAGATTCGGGTGGTGGTTTTAATCTGAGTT
TAGAGGAAATCGAAGAGGATGCGGAGGATGAGGTTCCAGATAATCCGTTTTTAAAAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X9N6B9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.932

90.076

0.504
  pilU Acinetobacter baylyi ADP1

52.528

90.585

0.476
  pilU Vibrio cholerae strain A1552

48.571

89.059

0.433
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.637

98.219

0.389

Multiple sequence alignment