Detailed information NTG0021440

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MCA_RS01600
• Genome accession: NC_002977
• Coordinates: 320665..321726 (+)
• Length: 353 a.a.
• NCBI ID: WP_017365267.1
• Uniprot ID: -
• Organism: Methylococcus capsulatus str. Bath
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 315665..326726

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MCA_RS01580 (MCA0321)	-	316142..316915 (-)	774	WP_010959682.1	exodeoxyribonuclease III	-
MCA_RS01585 (MCA0322)	rpmE	317030..317233 (+)	204	WP_010959683.1	50S ribosomal protein L31	-
MCA_RS01590 (MCA0323)	-	317269..317880 (-)	612	WP_010959684.1	hypothetical protein	-
MCA_RS01595 (MCA0324)	-	317896..320343 (-)	2448	WP_228370247.1	penicillin-binding protein 1A	-
MCA_RS01600 (MCA0325)	pilM	320665..321726 (+)	1062	WP_017365267.1	pilus assembly protein PilM	Machinery gene
MCA_RS01605 (MCA0326)	-	321726..322331 (+)	606	WP_017365266.1	PilN domain-containing protein	-
MCA_RS01610 (MCA0327)	-	322328..322966 (+)	639	WP_010959688.1	type 4a pilus biogenesis protein PilO	-
MCA_RS01615 (MCA0328)	-	322966..323508 (+)	543	WP_010959689.1	pilus assembly protein PilP	-
MCA_RS01620 (MCA0329)	pilQ	323624..325684 (+)	2061	WP_228370326.1	type IV pilus secretin PilQ	-
MCA_RS01625 (MCA0330)	aroK	325746..326285 (+)	540	WP_010959691.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 38425.92 Da
• Isoelectric Point: 4.9350

>NTDB_id=21440 MCA_RS01600 WP_017365267.1 320665..321726(+) (pilM) [Methylococcus capsulatus str. Bath]
MWGLGRKKPLLLGIDISAAAVKLLELSRKNGGYQVESYGVVPLPRNTVVDNTLAEPDNVSAAVRAVVKQSGTALRHAVVA
VPGSVVITKRIALPANLEGDELEAQIELEADQYIPHAREEVSLDFEVLGKNPKNPDLLDVLLVATRRENVEDRVSVLENA
GLGVEIVDVESYAIERAFELVRDQLPPLLRDRAVAVADVGAMATTLNVIHGGSIIYTREQGFGGMQLTDEIQRRYGLTYE
EAGLAKKEGSLPGNYAEEVLEPFKHALVQQISRSFQFYLSSTAQRGFDAVVLAGGCAMIPGIDRYVEAALQVPTVIANPF
RHMSFSGRVRQERLSYDSSALMLACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=21440 MCA_RS01600 WP_017365267.1 320665..321726(+) (pilM) [Methylococcus capsulatus str. Bath]
ATGTGGGGCCTAGGAAGGAAGAAACCGCTCCTGCTGGGGATCGACATCAGCGCGGCGGCGGTCAAGCTGCTCGAGCTGAG
CAGAAAGAACGGCGGCTACCAGGTGGAAAGCTATGGCGTTGTGCCTTTGCCCCGCAACACGGTGGTCGACAACACCCTTG
CCGAGCCGGACAACGTGTCGGCGGCGGTCCGGGCCGTGGTCAAACAGTCGGGGACCGCGCTCAGGCACGCGGTGGTCGCG
GTGCCCGGCTCGGTGGTCATCACCAAGCGCATCGCCCTACCCGCCAACCTGGAGGGCGACGAACTCGAGGCGCAGATCGA
ACTGGAGGCGGACCAGTACATTCCTCACGCCAGGGAGGAAGTCAGCCTGGATTTCGAGGTACTGGGGAAGAATCCGAAGA
ATCCCGATCTGCTGGATGTGCTGCTGGTGGCGACCCGCCGGGAGAACGTGGAAGACCGGGTCTCGGTACTGGAAAATGCC
GGACTCGGCGTCGAGATCGTCGATGTCGAGTCCTATGCCATCGAACGCGCCTTCGAACTTGTCCGGGATCAGCTTCCACC
GCTCCTCCGCGACCGGGCGGTTGCCGTAGCCGATGTGGGCGCCATGGCCACGACGCTCAACGTCATCCATGGCGGTTCCA
TCATCTACACGCGGGAACAGGGTTTCGGCGGCATGCAGCTCACCGACGAGATCCAGCGGCGCTACGGTTTGACCTACGAG
GAAGCGGGGCTGGCGAAGAAGGAGGGGAGCCTTCCCGGCAACTATGCCGAAGAGGTGCTGGAGCCGTTCAAGCATGCCTT
GGTTCAGCAGATCAGCCGATCGTTCCAGTTCTATCTGTCGTCCACTGCGCAGCGTGGTTTCGATGCGGTGGTGCTGGCCG
GCGGCTGCGCCATGATTCCGGGGATAGACCGTTACGTCGAGGCGGCGTTGCAGGTTCCCACCGTGATTGCCAATCCCTTC
CGTCACATGAGTTTTTCGGGAAGGGTGCGGCAGGAGCGCCTGAGTTACGATTCATCCGCCCTGATGCTGGCATGCGGCCT
GGCACTGCGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.603	97.167	0.501
comM	Acinetobacter nosocomialis M2	51.02	97.167	0.496
comM	Acinetobacter baylyi ADP1	49.138	98.584	0.484
pilM	Legionella pneumophila strain ERS1305867	42.733	97.45	0.416