Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   BRL61_RS08025 Genome accession   NZ_CP019117
Coordinates   1605521..1606018 (-) Length   165 a.a.
NCBI ID   WP_001794328.1    Uniprot ID   -
Organism   Staphylococcus aureus strain SJTUF_J27     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1600521..1611018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRL61_RS08000 (BRL61_07995) gcvPB 1600683..1602155 (-) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  BRL61_RS08005 (BRL61_08000) gcvPA 1602148..1603494 (-) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  BRL61_RS08010 (BRL61_08005) gcvT 1603514..1604605 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  BRL61_RS08015 (BRL61_08010) - 1604764..1605288 (-) 525 WP_001015125.1 shikimate kinase -
  BRL61_RS08020 (BRL61_08015) - 1605278..1605424 (-) 147 WP_001789879.1 hypothetical protein -
  BRL61_RS08025 (BRL61_08020) comGF 1605521..1606018 (-) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  BRL61_RS08030 (BRL61_08025) comGE 1605936..1606235 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  BRL61_RS08035 (BRL61_08030) comGD 1606222..1606668 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  BRL61_RS08040 (BRL61_08035) comGC 1606646..1606957 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  BRL61_RS08045 (BRL61_08040) comGB 1606971..1608041 (-) 1071 WP_053865724.1 competence type IV pilus assembly protein ComGB Machinery gene
  BRL61_RS08050 (BRL61_08045) comGA 1608013..1608987 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  BRL61_RS08055 (BRL61_08050) - 1609039..1609662 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  BRL61_RS08060 (BRL61_08055) - 1609659..1609988 (-) 330 WP_053865723.1 MTH1187 family thiamine-binding protein -
  BRL61_RS08065 (BRL61_08060) - 1609988..1610974 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19197.95 Da        Isoelectric Point: 10.2192

>NTDB_id=213227 BRL61_RS08025 WP_001794328.1 1605521..1606018(-) (comGF) [Staphylococcus aureus strain SJTUF_J27]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=213227 BRL61_RS08025 WP_001794328.1 1605521..1606018(-) (comGF) [Staphylococcus aureus strain SJTUF_J27]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

97.576

100

0.976

Multiple sequence alignment