Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BRL61_RS08050 Genome accession   NZ_CP019117
Coordinates   1608013..1608987 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain SJTUF_J27     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1603013..1613987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRL61_RS08010 (BRL61_08005) gcvT 1603514..1604605 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  BRL61_RS08015 (BRL61_08010) - 1604764..1605288 (-) 525 WP_001015125.1 shikimate kinase -
  BRL61_RS08020 (BRL61_08015) - 1605278..1605424 (-) 147 WP_001789879.1 hypothetical protein -
  BRL61_RS08025 (BRL61_08020) comGF 1605521..1606018 (-) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  BRL61_RS08030 (BRL61_08025) comGE 1605936..1606235 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  BRL61_RS08035 (BRL61_08030) comGD 1606222..1606668 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  BRL61_RS08040 (BRL61_08035) comGC 1606646..1606957 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  BRL61_RS08045 (BRL61_08040) comGB 1606971..1608041 (-) 1071 WP_053865724.1 competence type IV pilus assembly protein ComGB Machinery gene
  BRL61_RS08050 (BRL61_08045) comGA 1608013..1608987 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  BRL61_RS08055 (BRL61_08050) - 1609039..1609662 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  BRL61_RS08060 (BRL61_08055) - 1609659..1609988 (-) 330 WP_053865723.1 MTH1187 family thiamine-binding protein -
  BRL61_RS08065 (BRL61_08060) - 1609988..1610974 (-) 987 WP_000161314.1 ROK family glucokinase -
  BRL61_RS08070 (BRL61_08065) - 1610971..1611174 (-) 204 WP_000087561.1 YqgQ family protein -
  BRL61_RS08075 (BRL61_08070) - 1611155..1612618 (-) 1464 WP_001017867.1 rhomboid family protein -
  BRL61_RS08080 (BRL61_08075) - 1612630..1613169 (-) 540 WP_000164553.1 5-formyltetrahydrofolate cyclo-ligase -
  BRL61_RS08085 (BRL61_08080) rpmG 1613378..1613527 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=213232 BRL61_RS08050 WP_000697220.1 1608013..1608987(-) (comGA) [Staphylococcus aureus strain SJTUF_J27]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=213232 BRL61_RS08050 WP_000697220.1 1608013..1608987(-) (comGA) [Staphylococcus aureus strain SJTUF_J27]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTGCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment