Detailed information NTG0212663

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AT570_RS11680
• Genome accession: NZ_CP019034
• Coordinates: 2453355..2454473 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain AB042
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2448355..2459473

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AT570_RS11645 (AT570_11640)	-	2449184..2450032 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
AT570_RS11650 (AT570_11645)	-	2450285..2450740 (+)	456	WP_000782976.1	bacteriohemerythrin	-
AT570_RS11660 (AT570_11655)	-	2450893..2451960 (+)	1068	WP_004748097.1	hypothetical protein	-
AT570_RS11665 (AT570_11660)	-	2451960..2452283 (+)	324	WP_000442590.1	RnfH family protein	-
AT570_RS11670 (AT570_11665)	-	2452338..2452736 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AT570_RS11675 (AT570_11670)	fur	2452848..2453285 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AT570_RS11680 (AT570_11675)	pilU	2453355..2454473 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AT570_RS11685 (AT570_11680)	pilT	2454501..2455538 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AT570_RS11690 (AT570_11685)	-	2455665..2456357 (+)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=212663 AT570_RS11680 WP_000347034.1 2453355..2454473(-) (pilU) [Acinetobacter baumannii strain AB042]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=212663 AT570_RS11680 WP_000347034.1 2453355..2454473(-) (pilU) [Acinetobacter baumannii strain AB042]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39