Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NGO_RS08290 Genome accession   NC_002946
Coordinates   1627559..1629235 (-) Length   558 a.a.
NCBI ID   WP_010951320.1    Uniprot ID   Q5F689
Organism   Neisseria gonorrhoeae FA 1090     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1622559..1634235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGO_RS08270 (NGO1669) pilG 1624476..1625708 (+) 1233 WP_003689811.1 type 4 pilus assembly protein PilG Machinery gene
  NGO_RS08275 (NGO1670) pilD 1625782..1626642 (+) 861 WP_003689814.1 prepilin peptidase Machinery gene
  NGO_RS08280 (NGO1671) coaE 1626639..1627271 (+) 633 WP_003689816.1 dephospho-CoA kinase -
  NGO_RS08285 (NGO1672) - 1627264..1627473 (+) 210 WP_003696096.1 DNA gyrase inhibitor YacG -
  NGO_RS08290 (NGO1673) pilF 1627559..1629235 (-) 1677 WP_010951320.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NGO_RS08300 (NGO1674) - 1629573..1630019 (-) 447 WP_003689818.1 DUF441 domain-containing protein -
  NGO_RS08305 (NGO1675) - 1630023..1630997 (-) 975 WP_003689819.1 polyprenyl synthetase family protein -
  NGO_RS08315 (NGO1676) rplU 1631221..1631529 (+) 309 WP_003691838.1 50S ribosomal protein L21 -
  NGO_RS08320 (NGO1677) rpmA 1631554..1631826 (+) 273 WP_003689821.1 50S ribosomal protein L27 -
  NGO_RS08325 (NGO1678) ubiM 1632061..1633245 (+) 1185 WP_010951321.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  NGO_RS08330 (NGO1679) rpmG 1633434..1633589 (-) 156 WP_003689824.1 50S ribosomal protein L33 -
  NGO_RS08335 (NGO1680) rpmB 1633621..1633854 (-) 234 WP_002216391.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61891.06 Da        Isoelectric Point: 5.6921

>NTDB_id=21167 NGO_RS08290 WP_010951320.1 1627559..1629235(-) (pilF) [Neisseria gonorrhoeae FA 1090]
MSVGLLRILVQNQVVTVERAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=21167 NGO_RS08290 WP_010951320.1 1627559..1629235(-) (pilF) [Neisseria gonorrhoeae FA 1090]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCGGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACCCAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGCGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAAGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGTAGCGG
GGCATCCGACATCCATTTCGAGTTTTACGAACACAATGCGCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGTAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTCCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCTTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACACTGCACACGAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGTTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGGTATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAATTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5F689

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.821

100

0.998
  pilB Acinetobacter baumannii D1279779

50.088

100

0.509
  pilB Acinetobacter baylyi ADP1

50.178

100

0.505
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.459
  pilB Vibrio cholerae strain A1552

45.196

100

0.455
  pilB Vibrio campbellii strain DS40M4

43.662

100

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.885

93.907

0.375

Multiple sequence alignment