Detailed information NTG0021072

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: HD_RS04625
• Genome accession: NC_002940
• Coordinates: 892515..892964 (+)
• Length: 149 a.a.
• NCBI ID: WP_010945035.1
• Uniprot ID: -
• Organism: [Haemophilus] ducreyi 35000HP
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 887515..897964

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HD_RS08480 (HD1112)	-	887645..887755 (-)	111	Protein_881	formate dehydrogenase N subunit beta transmembrane domain-containing protein	-
HD_RS04595 (HD1113)	fdhD	887756..888442 (+)	687	Protein_882	formate dehydrogenase accessory sulfurtransferase FdhD	-
HD_RS04600 (HD1115)	nth	888520..889155 (-)	636	WP_010945029.1	endonuclease III	-
HD_RS04605 (HD1117)	-	889185..889934 (-)	750	WP_041603673.1	TSUP family transporter	-
HD_RS04610 (HD1118)	-	890053..890538 (+)	486	WP_010945031.1	dihydrofolate reductase	-
HD_RS04615 (HD1120)	-	890660..890875 (+)	216	WP_010945033.1	YgjV family protein	-
HD_RS04620 (HD1121)	radA	890942..892321 (-)	1380	WP_010945034.1	DNA repair protein RadA	-
HD_RS04625 (HD1123)	pilA	892515..892964 (+)	450	WP_010945035.1	prepilin peptidase-dependent pilin	Machinery gene
HD_RS04630 (HD1124)	pilB	892976..894370 (+)	1395	WP_010945036.1	GspE/PulE family protein	Machinery gene
HD_RS04635 (HD1125)	pilC	894354..895547 (+)	1194	WP_010945037.1	type II secretion system F family protein	Machinery gene
HD_RS04640 (HD1126)	-	895547..896239 (+)	693	WP_041603480.1	prepilin peptidase	-
HD_RS04645 (HD1127)	coaE	896257..896874 (+)	618	WP_010945039.1	dephospho-CoA kinase	-
HD_RS04650 (HD1129)	yacG	896877..897065 (+)	189	WP_010945040.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 16256.69 Da
• Isoelectric Point: 6.9012

>NTDB_id=21072 HD_RS04625 WP_010945035.1 892515..892964(+) (pilA) [[Haemophilus] ducreyi 35000HP]
MSSLFLIKPLQKAFTLIELMIVIAIIAILATVAIPSYNSYTKKAALSELLAASSAYKTDVEICVYNLGRTDGCNNGENGI
KNDKLDKSKYVASVAVENGEITVKGKESLKDYSYTMKPELQQDGHLTWKTTCNGADTSLFPANFCSTKE

Nucleotide

• Length: 450 bp

>NTDB_id=21072 HD_RS04625 WP_010945035.1 892515..892964(+) (pilA) [[Haemophilus] ducreyi 35000HP]
ATGTCATCTTTATTTTTGATTAAGCCACTACAAAAAGCGTTTACTTTAATTGAATTAATGATTGTGATTGCCATTATCGC
AATTTTAGCCACGGTTGCGATTCCTTCTTACAATAGTTATACCAAAAAAGCTGCGTTATCTGAGTTATTAGCCGCTTCAT
CAGCGTATAAAACCGATGTAGAAATATGTGTTTATAATCTTGGTAGAACGGATGGATGCAATAATGGAGAAAATGGTATA
AAAAATGATAAATTAGATAAAAGTAAATATGTTGCTTCAGTTGCGGTGGAAAATGGAGAAATTACAGTAAAAGGTAAAGA
AAGCCTTAAAGATTATAGTTACACAATGAAGCCAGAACTACAACAAGATGGGCACTTAACTTGGAAAACGACTTGTAATG
GTGCTGATACTAGCCTTTTCCCTGCTAATTTTTGTTCTACTAAAGAGTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (11-33)

• Pilin (bacterial filament) (Pilin) (43-143)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Glaesserella parasuis strain SC1401	59.868	100	0.611
pilA	Haemophilus influenzae Rd KW20	56.25	96.644	0.544
pilA	Haemophilus influenzae 86-028NP	57.143	93.96	0.537