Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   BBX44_RS20350 Genome accession   NZ_CP024024
Coordinates   4317507..4318130 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PARM801     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4312507..4323130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBX44_RS20330 aroB 4313102..4314208 (-) 1107 WP_003114574.1 3-dehydroquinate synthase -
  BBX44_RS20335 aroK 4314258..4314776 (-) 519 WP_003095833.1 shikimate kinase AroK -
  BBX44_RS20340 pilQ 4314788..4316932 (-) 2145 WP_049272019.1 type 4a pilus secretin PilQ Machinery gene
  BBX44_RS20345 comL/pilP 4316986..4317510 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  BBX44_RS20350 pilO 4317507..4318130 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  BBX44_RS20355 pilN 4318127..4318723 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  BBX44_RS20360 pilM 4318723..4319787 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  BBX44_RS20365 - 4319972..4322443 (+) 2472 WP_049272020.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=209735 BBX44_RS20350 WP_003103268.1 4317507..4318130(-) (pilO) [Pseudomonas aeruginosa strain PARM801]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=209735 BBX44_RS20350 WP_003103268.1 4317507..4318130(-) (pilO) [Pseudomonas aeruginosa strain PARM801]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401

Multiple sequence alignment