Detailed information NTG0209606

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BBX32_RS06940
• Genome accession: NZ_CP018677
• Coordinates: 1418615..1419733 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347035.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain LAC4
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1413615..1424733

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BBX32_RS06905 (BBX32_06875)	-	1414444..1415292 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
BBX32_RS06910 (BBX32_06880)	-	1415545..1416000 (+)	456	WP_000782976.1	bacteriohemerythrin	-
BBX32_RS06920 (BBX32_06885)	-	1416153..1417220 (+)	1068	WP_005120426.1	hypothetical protein	-
BBX32_RS06925 (BBX32_06890)	-	1417220..1417543 (+)	324	WP_000442585.1	RnfH family protein	-
BBX32_RS06930 (BBX32_06895)	-	1417598..1417996 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
BBX32_RS06935 (BBX32_06900)	fur	1418108..1418545 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
BBX32_RS06940 (BBX32_06905)	pilU	1418615..1419733 (-)	1119	WP_000347035.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BBX32_RS06945 (BBX32_06910)	pilT	1419761..1420798 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
BBX32_RS06950 (BBX32_06915)	-	1420925..1421617 (+)	693	WP_001108510.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41821.98 Da
• Isoelectric Point: 6.5675

>NTDB_id=209606 BBX32_RS06940 WP_000347035.1 1418615..1419733(-) (pilU) [Acinetobacter baumannii strain LAC4]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDSQ

Nucleotide

• Length: 1119 bp

>NTDB_id=209606 BBX32_RS06940 WP_000347035.1 1418615..1419733(-) (pilU) [Acinetobacter baumannii strain LAC4]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACAGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.817	100	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39