Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   CRN68_RS08485 Genome accession   NZ_CP023960
Coordinates   1376376..1377386 (-) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain FDAARGOS_448     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1371376..1382386
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRN68_RS08465 (CRN68_08465) mepM 1372340..1373662 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  CRN68_RS08470 (CRN68_08470) znuA 1373678..1374610 (-) 933 WP_011076428.1 zinc ABC transporter substrate-binding protein ZnuA -
  CRN68_RS08475 (CRN68_08475) znuC 1374689..1375444 (+) 756 WP_000202987.1 zinc ABC transporter ATP-binding protein ZnuC -
  CRN68_RS08480 (CRN68_08480) znuB 1375441..1376226 (+) 786 WP_001520716.1 zinc ABC transporter permease subunit ZnuB -
  CRN68_RS08485 (CRN68_08485) ruvB 1376376..1377386 (-) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  CRN68_RS08490 (CRN68_08490) ruvA 1377395..1378006 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  CRN68_RS08495 (CRN68_08495) yobI 1378145..1378210 (-) 66 WP_072146566.1 stress response small protein YobI -
  CRN68_RS08500 (CRN68_08500) yebB 1378281..1378883 (+) 603 WP_001520719.1 YebB family permuted papain-like enzyme -
  CRN68_RS08505 (CRN68_08505) ruvC 1378885..1379406 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  CRN68_RS08510 (CRN68_08510) yebC 1379441..1380181 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  CRN68_RS08515 (CRN68_08515) nudB 1380210..1380662 (-) 453 WP_001308712.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=209472 CRN68_RS08485 WP_000568522.1 1376376..1377386(-) (ruvB) [Escherichia coli strain FDAARGOS_448]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=209472 CRN68_RS08485 WP_000568522.1 1376376..1377386(-) (ruvB) [Escherichia coli strain FDAARGOS_448]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCTAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGGGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACACTGATTGGCGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTACGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCTAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment