Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   BS467_RS12570 Genome accession   NZ_CP018574
Coordinates   2413613..2413906 (+) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus altitudinis strain GLB197     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2408613..2418906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS467_RS12540 (BS467_12545) - 2409109..2409351 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  BS467_RS12545 (BS467_12550) - 2409564..2409944 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BS467_RS12555 (BS467_12560) - 2410170..2411039 (+) 870 WP_007501231.1 STAS domain-containing protein -
  BS467_RS12560 (BS467_12565) comGA 2411505..2412575 (+) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  BS467_RS12565 (BS467_12570) comGB 2412556..2413596 (+) 1041 WP_017357986.1 competence type IV pilus assembly protein ComGB -
  BS467_RS12570 (BS467_12575) comGC 2413613..2413906 (+) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  BS467_RS12575 (BS467_12580) comGD 2413899..2414345 (+) 447 WP_017357985.1 competence type IV pilus minor pilin ComGD -
  BS467_RS12580 (BS467_12585) comGE 2414329..2414643 (+) 315 WP_017357984.1 competence type IV pilus minor pilin ComGE -
  BS467_RS12585 (BS467_12590) comGF 2414585..2415082 (+) 498 WP_041091745.1 competence type IV pilus minor pilin ComGF -
  BS467_RS12590 (BS467_12595) - 2415079..2415462 (+) 384 WP_041091746.1 competence type IV pilus minor pilin ComGG -
  BS467_RS12595 (BS467_12600) - 2415523..2415717 (+) 195 WP_008342191.1 YqzE family protein -
  BS467_RS12600 (BS467_12605) - 2415752..2416078 (-) 327 WP_007501224.1 DUF3889 domain-containing protein -
  BS467_RS12605 (BS467_12610) tapA 2416331..2416858 (+) 528 WP_041091748.1 amyloid fiber anchoring/assembly protein TapA -
  BS467_RS12610 (BS467_12615) sipW 2416908..2417480 (+) 573 WP_041091749.1 signal peptidase I SipW -
  BS467_RS12615 (BS467_12620) tasA 2417539..2418339 (+) 801 WP_008342195.1 biofilm matrix protein TasA -
  BS467_RS12620 (BS467_12625) sinR 2418407..2418742 (-) 336 WP_017357978.1 transcriptional regulator SinR Regulator

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=208993 BS467_RS12570 WP_008342186.1 2413613..2413906(+) (comGC) [Bacillus altitudinis strain GLB197]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=208993 BS467_RS12570 WP_008342186.1 2413613..2413906(+) (comGC) [Bacillus altitudinis strain GLB197]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTTATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCAAATGGAAAAGCAATTTTCATTCAAAACGGCGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment