Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   CRT46_RS10525 Genome accession   NZ_CP023820
Coordinates   2139171..2140181 (-) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain 7/2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2134171..2145181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRT46_RS10505 (CRT46_11035) mepM 2135135..2136457 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  CRT46_RS10510 (CRT46_11040) znuA 2136473..2137405 (-) 933 WP_011076428.1 zinc ABC transporter substrate-binding protein ZnuA -
  CRT46_RS10515 (CRT46_11045) znuC 2137484..2138239 (+) 756 WP_000202987.1 zinc ABC transporter ATP-binding protein ZnuC -
  CRT46_RS10520 (CRT46_11050) znuB 2138236..2139021 (+) 786 WP_001520716.1 zinc ABC transporter permease subunit ZnuB -
  CRT46_RS10525 (CRT46_11055) ruvB 2139171..2140181 (-) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  CRT46_RS10530 (CRT46_11060) ruvA 2140190..2140801 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  CRT46_RS10535 (CRT46_11065) yobI 2140940..2141005 (-) 66 WP_072146566.1 stress response small protein YobI -
  CRT46_RS10540 (CRT46_11070) yebB 2141076..2141678 (+) 603 WP_001520719.1 YebB family permuted papain-like enzyme -
  CRT46_RS10545 (CRT46_11075) ruvC 2141680..2142201 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  CRT46_RS10550 (CRT46_11080) yebC 2142236..2142976 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  CRT46_RS10555 (CRT46_11085) nudB 2143005..2143457 (-) 453 WP_001308712.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=208799 CRT46_RS10525 WP_000568522.1 2139171..2140181(-) (ruvB) [Escherichia coli strain 7/2]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=208799 CRT46_RS10525 WP_000568522.1 2139171..2140181(-) (ruvB) [Escherichia coli strain 7/2]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCTAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGGGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACACTGATTGGCGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTACGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCTAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment