Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   PM_RS06370 Genome accession   NC_002663
Coordinates   1416502..1417836 (-) Length   444 a.a.
NCBI ID   WP_010907082.1    Uniprot ID   Q9CLK3
Organism   Pasteurella multocida subsp. multocida str. Pm70     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1411502..1422836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PM_RS06345 (PM1220) secM 1413022..1413333 (-) 312 WP_005723689.1 secA translation cis-regulator SecM -
  PM_RS06350 (PM1221) - 1413426..1413731 (+) 306 WP_005723691.1 DUF721 domain-containing protein -
  PM_RS06355 (PM1222) - 1413778..1414683 (-) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  PM_RS06360 (PM1223) aroB 1414687..1415775 (-) 1089 WP_010907081.1 3-dehydroquinate synthase -
  PM_RS06365 (PM1224) aroK 1415792..1416319 (-) 528 WP_005717623.1 shikimate kinase AroK -
  PM_RS06370 (PM1225) comE 1416502..1417836 (-) 1335 WP_010907082.1 type IV pilus secretin PilQ Machinery gene
  PM_RS06375 (PM1226) - 1417846..1418208 (-) 363 WP_005748555.1 pilus assembly protein PilP -
  PM_RS06380 (PM1227) - 1418211..1418735 (-) 525 WP_005754754.1 hypothetical protein -
  PM_RS06385 (PM1228) - 1418735..1419250 (-) 516 WP_005751904.1 hypothetical protein -
  PM_RS06390 (PM1229) - 1419290..1420063 (-) 774 WP_010907083.1 pilus assembly protein PilM -
  PM_RS06395 (PM1230) - 1420199..1422760 (+) 2562 WP_010907084.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49206.63 Da        Isoelectric Point: 8.0315

>NTDB_id=20794 PM_RS06370 WP_010907082.1 1416502..1417836(-) (comE) [Pasteurella multocida subsp. multocida str. Pm70]
MWRAFRKISLVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=20794 PM_RS06370 WP_010907082.1 1416502..1417836(-) (comE) [Pasteurella multocida subsp. multocida str. Pm70]
ATGTGGCGAGCATTCAGAAAAATATCTCTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCACTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGTGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CLK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.212

97.748

0.608
  comE Haemophilus influenzae 86-028NP

61.521

97.748

0.601
  comE Glaesserella parasuis strain SC1401

50.976

92.342

0.471
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369

Multiple sequence alignment