Detailed information NTG0207743

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BS065_RS14620
• Genome accession: NZ_CP018256
• Coordinates: 3020293..3021411 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain AF-673
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3015293..3026411

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BS065_RS14610 (BS065_14605)	-	3018409..3019101 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
BS065_RS14615 (BS065_14610)	pilT	3019228..3020265 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
BS065_RS14620 (BS065_14615)	pilU	3020293..3021411 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BS065_RS14625 (BS065_14620)	fur	3021481..3021918 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
BS065_RS14630 (BS065_14625)	-	3022030..3022428 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
BS065_RS14635 (BS065_14630)	-	3022483..3022806 (-)	324	WP_000442587.1	RnfH family protein	-
BS065_RS14640 (BS065_14635)	-	3022806..3023873 (-)	1068	WP_002019572.1	hypothetical protein	-
BS065_RS14650 (BS065_14645)	-	3024026..3024481 (-)	456	WP_000782976.1	bacteriohemerythrin	-
BS065_RS14655 (BS065_14650)	-	3024734..3025582 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=207743 BS065_RS14620 WP_000347039.1 3020293..3021411(+) (pilU) [Acinetobacter baumannii strain AF-673]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=207743 BS065_RS14620 WP_000347039.1 3020293..3021411(+) (pilU) [Acinetobacter baumannii strain AF-673]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395