Detailed information NTG0207714

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BS065_RS01515
• Genome accession: NZ_CP018256
• Coordinates: 316584..317642 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain AF-673
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 311584..322642

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BS065_RS01500 (BS065_01485)	-	312689..313021 (+)	333	WP_000169594.1	DUF7709 family protein	-
BS065_RS01505 (BS065_01490)	-	313024..313848 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
BS065_RS01510 (BS065_01495)	ponA	313866..316421 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
BS065_RS01515 (BS065_01500)	pilM	316584..317642 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
BS065_RS01520 (BS065_01505)	pilN	317642..318283 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
BS065_RS01525 (BS065_01510)	pilO	318280..319020 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
BS065_RS01530 (BS065_01515)	pilP	319031..319558 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
BS065_RS01535 (BS065_01520)	pilQ	319626..321791 (+)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
BS065_RS01540 (BS065_01525)	aroK	321803..322372 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=207714 BS065_RS01515 WP_002014066.1 316584..317642(+) (pilM) [Acinetobacter baumannii strain AF-673]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=207714 BS065_RS01515 WP_002014066.1 316584..317642(+) (pilM) [Acinetobacter baumannii strain AF-673]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423