Detailed information NTG0207201

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: JN853_RS04400
• Genome accession: NZ_CP018202
• Coordinates: 870811..871875 (+)
• Length: 354 a.a.
• NCBI ID: WP_003378852.1
• Uniprot ID: A0A2G9KZJ9
• Organism: Pseudomonas syringae pv. actinidiae ICMP 9853
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 865811..876875

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JN853_RS04390 (JN853_04320)	-	866567..867835 (+)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-
JN853_RS04395 (JN853_04325)	-	868161..870602 (-)	2442	WP_375233189.1	penicillin-binding protein 1A	-
JN853_RS04400 (JN853_04330)	pilM	870811..871875 (+)	1065	WP_003378852.1	pilus assembly protein PilM	Machinery gene
JN853_RS04405 (JN853_04335)	-	871875..872501 (+)	627	WP_003378851.1	PilN domain-containing protein	-
JN853_RS04410 (JN853_04340)	pilO	872498..873121 (+)	624	WP_003378850.1	type 4a pilus biogenesis protein PilO	-
JN853_RS04415 (JN853_04345)	pilP	873118..873645 (+)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
JN853_RS04420 (JN853_04350)	pilQ	873693..875798 (+)	2106	WP_003378848.1	type IV pilus secretin PilQ	Machinery gene
JN853_RS04425 (JN853_04355)	aroK	875803..876321 (+)	519	WP_003378847.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38103.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=207201 JN853_RS04400 WP_003378852.1 870811..871875(+) (pilM) [Pseudomonas syringae pv. actinidiae ICMP 9853]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=207201 JN853_RS04400 WP_003378852.1 870811..871875(+) (pilM) [Pseudomonas syringae pv. actinidiae ICMP 9853]
GTGTTCGAACTCTTTGGCAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCTCGCGTACTGGTCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
TAAGCTTGAAGCTGATCAGTACATCCCTTATCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGTTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTC
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGGCTTCTGGCTGCGCAACTGGG
CAACGGTCATGACGAACTGACCGTTGCCGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCACGGCC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTGACTGACGAAATTCAGCGTCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAAGTGCTGGACCCCTTCAAGGACGC
GCTCGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACGCCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGCTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2G9KZJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469