Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   JN853_RS02700 Genome accession   NZ_CP018202
Coordinates   520229..520798 (+) Length   189 a.a.
NCBI ID   WP_003426596.1    Uniprot ID   A0A0K8M6E3
Organism   Pseudomonas syringae pv. actinidiae ICMP 9853     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 520229..537878 520229..520798 within 0
IScluster/Tn 520970..522549 520229..520798 flank 172


Gene organization within MGE regions


Location: 520229..537878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN853_RS02700 (JN853_02660) ssb 520229..520798 (+) 570 WP_003426596.1 single-stranded DNA-binding protein Machinery gene
  JN853_RS02715 (JN853_02675) - 522612..523829 (+) 1218 WP_004397021.1 tyrosine-type recombinase/integrase -
  JN853_RS02720 (JN853_02680) - 523826..525352 (+) 1527 WP_004397020.1 site-specific integrase -
  JN853_RS02725 (JN853_02685) - 525345..527354 (+) 2010 WP_017699151.1 hypothetical protein -
  JN853_RS02730 (JN853_02690) - 527347..527760 (+) 414 WP_003381885.1 hypothetical protein -
  JN853_RS02735 (JN853_02695) tnpC 527976..529508 (-) 1533 WP_077142724.1 IS66 family transposase -
  JN853_RS02740 (JN853_02700) tnpB 529526..529885 (-) 360 WP_005621010.1 IS66 family insertion sequence element accessory protein TnpB -
  JN853_RS32575 - 529946..530398 (+) 453 WP_019334430.1 hypothetical protein -
  JN853_RS02745 (JN853_02705) - 530405..531472 (-) 1068 WP_077142725.1 IS630-like element ISPsy32 family transposase -
  JN853_RS02750 (JN853_02710) - 531708..532775 (-) 1068 WP_077142726.1 IS630-like element ISPsy32 family transposase -
  JN853_RS02755 (JN853_02715) - 533485..534552 (+) 1068 WP_032685473.1 IS630-like element ISPsy32 family transposase -
  JN853_RS02760 (JN853_02720) - 534842..535141 (-) 300 WP_003379193.1 hypothetical protein -
  JN853_RS02765 (JN853_02725) - 535264..536007 (-) 744 WP_003379192.1 inositol monophosphatase family protein -
  JN853_RS02770 (JN853_02730) - 536004..536765 (-) 762 WP_003379189.1 glycerophosphoryl diester phosphodiesterase -
  JN853_RS02775 (JN853_02735) - 536772..537878 (-) 1107 WP_003379187.1 ABC transporter ATP-binding protein -

Sequence


Protein


Download         Length: 189 a.a.        Molecular weight: 20974.10 Da        Isoelectric Point: 5.9404

>NTDB_id=207197 JN853_RS02700 WP_003426596.1 520229..520798(+) (ssb) [Pseudomonas syringae pv. actinidiae ICMP 9853]
MARGVNKVILVGTCGQDPEVRYLPNGNAVTNLSLATSEQWTDKQSGQKVEKTEWHRVSMFGKVAEIAGEYLRKGSQVYIE
GKLQTREWEKDGIKRYTTEIVVDMQGTMQLLGGRPQGDAQQGQGGGGNYNQSAPRPQQSRPQQSAPQQSAPQQNYNQQPP
QQRDSRPAPQQQAPQPAADFDSFDDDIPF

Nucleotide


Download         Length: 570 bp        

>NTDB_id=207197 JN853_RS02700 WP_003426596.1 520229..520798(+) (ssb) [Pseudomonas syringae pv. actinidiae ICMP 9853]
ATGGCCCGTGGGGTTAACAAAGTCATATTGGTCGGTACATGCGGCCAGGATCCCGAAGTTCGCTACTTGCCTAACGGTAA
CGCCGTGACCAACCTGAGTCTGGCAACCAGCGAACAATGGACTGACAAGCAGAGCGGCCAGAAGGTCGAGAAGACCGAGT
GGCACCGTGTTTCGATGTTCGGCAAGGTTGCCGAAATCGCCGGCGAATACCTGCGCAAGGGTTCGCAGGTCTACATCGAA
GGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACTGAAATCGTCGTCGACATGCAAGGCAC
CATGCAACTGCTGGGCGGCCGTCCACAGGGCGACGCTCAACAAGGTCAGGGCGGTGGCGGCAACTACAACCAGTCTGCAC
CTCGTCCACAGCAGTCGCGCCCACAGCAATCGGCGCCGCAGCAATCTGCCCCTCAGCAGAACTACAACCAGCAGCCGCCA
CAACAGCGCGATTCGCGCCCAGCGCCGCAACAACAAGCGCCGCAGCCTGCTGCTGACTTTGACAGCTTTGATGACGATAT
TCCGTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0K8M6E3

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

55.208

100

0.561

  ssb Glaesserella parasuis strain SC1401

47.368

100

0.476

  ssb Neisseria meningitidis MC58

44.681

99.471

0.444

  ssb Neisseria gonorrhoeae MS11

44.681

99.471

0.444


Multiple sequence alignment