Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   BSO20_RS00225 Genome accession   NZ_CP018200
Coordinates   36151..36588 (+) Length   145 a.a.
NCBI ID   WP_007612572.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain WS-8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 31151..41588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSO20_RS00200 (BSO20_00200) - 31160..32461 (-) 1302 WP_032874010.1 hemolysin family protein -
  BSO20_RS00205 (BSO20_00205) - 32607..33557 (+) 951 WP_032874012.1 magnesium transporter CorA family protein -
  BSO20_RS00210 (BSO20_00210) comGA 33754..34824 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  BSO20_RS00215 (BSO20_00215) comGB 34811..35848 (+) 1038 WP_032874014.1 competence type IV pilus assembly protein ComGB Machinery gene
  BSO20_RS00220 (BSO20_00220) comGC 35895..36161 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  BSO20_RS00225 (BSO20_00225) comGD 36151..36588 (+) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  BSO20_RS00230 (BSO20_00230) comGE 36572..36886 (+) 315 WP_032874016.1 competence type IV pilus minor pilin ComGE Machinery gene
  BSO20_RS00235 (BSO20_00235) comGF 36795..37295 (+) 501 WP_223204419.1 competence type IV pilus minor pilin ComGF -
  BSO20_RS00240 (BSO20_00240) comGG 37296..37673 (+) 378 WP_032874019.1 competence type IV pilus minor pilin ComGG Machinery gene
  BSO20_RS00245 (BSO20_00245) - 37730..37909 (+) 180 WP_022552966.1 YqzE family protein -
  BSO20_RS00250 (BSO20_00250) - 37950..38279 (-) 330 WP_032874021.1 DUF3889 domain-containing protein -
  BSO20_RS00255 (BSO20_00255) tapA 38538..39209 (+) 672 WP_032874023.1 amyloid fiber anchoring/assembly protein TapA -
  BSO20_RS00260 (BSO20_00260) sipW 39181..39765 (+) 585 WP_032874025.1 signal peptidase I SipW -
  BSO20_RS00265 (BSO20_00265) tasA 39830..40615 (+) 786 WP_032874027.1 biofilm matrix protein TasA -
  BSO20_RS00270 (BSO20_00270) sinR 40663..40998 (-) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  BSO20_RS00275 (BSO20_00275) sinI 41032..41205 (-) 174 WP_032874029.1 anti-repressor SinI Regulator

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16254.76 Da        Isoelectric Point: 10.1850

>NTDB_id=207097 BSO20_RS00225 WP_007612572.1 36151..36588(+) (comGD) [Bacillus amyloliquefaciens strain WS-8]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=207097 BSO20_RS00225 WP_007612572.1 36151..36588(+) (comGD) [Bacillus amyloliquefaciens strain WS-8]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAGGATTGTCGAGCGTTCTTTTGATTCGCTTCATATTACACTTGTAACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCAATTGAAGAGCGCCGGGTTCACCTATGAGATAACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

55.479

100

0.559

Multiple sequence alignment