Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BK055_RS12830 Genome accession   NZ_CP017775
Coordinates   2631300..2632370 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain 9912D     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2626300..2637370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BK055_RS12780 (BK055_12780) sipW 2626359..2626943 (-) 585 WP_014418370.1 signal peptidase I SipW -
  BK055_RS12785 (BK055_12785) tapA 2626915..2627586 (-) 672 WP_025649852.1 amyloid fiber anchoring/assembly protein TapA -
  BK055_RS12790 (BK055_12790) - 2627845..2628174 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  BK055_RS12795 (BK055_12795) - 2628215..2628394 (-) 180 WP_003153093.1 YqzE family protein -
  BK055_RS12800 (BK055_12800) comGG 2628451..2628828 (-) 378 WP_025649851.1 competence type IV pilus minor pilin ComGG Machinery gene
  BK055_RS12805 (BK055_12805) comGF 2628829..2629224 (-) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  BK055_RS12810 (BK055_12810) comGE 2629238..2629552 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  BK055_RS12815 (BK055_12815) comGD 2629536..2629973 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  BK055_RS12820 (BK055_12820) comGC 2629963..2630229 (-) 267 WP_050496152.1 competence type IV pilus major pilin ComGC Machinery gene
  BK055_RS12825 (BK055_12825) comGB 2630276..2631313 (-) 1038 WP_071181847.1 competence type IV pilus assembly protein ComGB Machinery gene
  BK055_RS12830 (BK055_12830) comGA 2631300..2632370 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  BK055_RS12835 (BK055_12835) - 2632563..2633513 (-) 951 WP_071181848.1 magnesium transporter CorA family protein -
  BK055_RS12840 (BK055_12840) - 2633659..2634960 (+) 1302 WP_071181849.1 hemolysin family protein -
  BK055_RS12845 (BK055_12845) - 2634998..2635828 (-) 831 WP_017418143.1 STAS domain-containing protein -
  BK055_RS12855 (BK055_12855) - 2636035..2636415 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BK055_RS12860 (BK055_12860) - 2636652..2636897 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=202821 BK055_RS12830 WP_012117985.1 2631300..2632370(-) (comGA) [Bacillus velezensis strain 9912D]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=202821 BK055_RS12830 WP_012117985.1 2631300..2632370(-) (comGA) [Bacillus velezensis strain 9912D]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTCCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment