Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AP3564_RS18535 Genome accession   NZ_CP017703
Coordinates   3741986..3743053 (-) Length   355 a.a.
NCBI ID   WP_063388093.1    Uniprot ID   A0A165XRN5
Organism   Aeribacillus pallidus strain KCTC3564     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3736986..3748053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AP3564_RS18495 (AP3564_18040) - 3737930..3738727 (+) 798 WP_063388101.1 YqhG family protein -
  AP3564_RS18500 (AP3564_18045) - 3738818..3738997 (-) 180 WP_063388100.1 YqzE family protein -
  AP3564_RS18505 (AP3564_18050) comGG 3739117..3739488 (-) 372 WP_063388099.1 competence type IV pilus minor pilin ComGG -
  AP3564_RS18510 (AP3564_18055) comGF 3739485..3739916 (-) 432 WP_157728011.1 competence type IV pilus minor pilin ComGF -
  AP3564_RS18515 (AP3564_18060) - 3739906..3740232 (-) 327 WP_063388097.1 hypothetical protein -
  AP3564_RS18520 (AP3564_18065) comGD 3740216..3740677 (-) 462 WP_063388096.1 competence type IV pilus minor pilin ComGD -
  AP3564_RS18525 (AP3564_18070) comGC 3740646..3740957 (-) 312 WP_063388095.1 competence type IV pilus major pilin ComGC Machinery gene
  AP3564_RS18530 (AP3564_18075) comGB 3740962..3741993 (-) 1032 WP_063388094.1 competence type IV pilus assembly protein ComGB -
  AP3564_RS18535 (AP3564_18080) comGA 3741986..3743053 (-) 1068 WP_063388093.1 competence type IV pilus ATPase ComGA Machinery gene
  AP3564_RS18540 (AP3564_18085) - 3743509..3744738 (-) 1230 WP_027725928.1 IS110 family transposase -
  AP3564_RS18550 (AP3564_18095) - 3745172..3745417 (+) 246 WP_063388092.1 DUF2626 domain-containing protein -
  AP3564_RS18555 (AP3564_18100) - 3745472..3746116 (-) 645 WP_094246208.1 MBL fold metallo-hydrolase -
  AP3564_RS18560 (AP3564_18105) - 3746363..3746536 (+) 174 WP_065095533.1 DUF2759 domain-containing protein -
  AP3564_RS18565 (AP3564_18110) - 3746652..3747770 (-) 1119 WP_157728013.1 hypothetical protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 40549.44 Da        Isoelectric Point: 8.6397

>NTDB_id=202016 AP3564_RS18535 WP_063388093.1 3741986..3743053(-) (comGA) [Aeribacillus pallidus strain KCTC3564]
MIEIEKLSEKLIELACSFSASDIHIVPKKKKAVIQFRIDDDIKEIFHLPKAICERLISHFKFMAQMDIGEKRRPQNGAYS
FHTIYGEVHLRLSTLPTVFDESLVIRILLEQSIQTPEKLSLFPSESKKMLSFLKHSHGLLILTGPTGSGKTTTLYSLLHY
AKKHFKRNIITLEDPVETKSDEMLQVQINEKAGITYANGLKAILRHDPDMIMVGEIRDHETARIAVRAGLSGHLVLTTMH
TRDAKGAIYRLLEFGVTFSEIEQTLIAVAAQRLVHLICPFCKGNCPPYCKKLKEIRRIGVYELLYGKNLTAAIREAKGEE
QSELDYMKLKDVIKKGIALGYLYPDIYDRWVFEYE

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=202016 AP3564_RS18535 WP_063388093.1 3741986..3743053(-) (comGA) [Aeribacillus pallidus strain KCTC3564]
ATGATTGAAATTGAAAAGCTTAGCGAAAAACTGATTGAACTGGCGTGTTCATTTAGTGCCTCAGACATCCATATTGTTCC
GAAAAAGAAAAAAGCAGTTATTCAATTTCGAATTGACGATGACATCAAAGAAATATTTCATCTACCAAAAGCTATTTGTG
AACGCTTAATTTCTCATTTTAAATTTATGGCCCAAATGGATATTGGCGAAAAAAGGCGTCCGCAAAACGGTGCTTACTCC
TTCCATACGATTTACGGAGAGGTGCATCTTCGTTTATCGACATTGCCTACAGTATTCGATGAAAGTCTAGTCATCCGAAT
ATTGCTAGAGCAATCGATTCAAACGCCTGAAAAACTTTCATTGTTCCCGTCAGAATCCAAAAAAATGCTTTCATTTTTAA
AGCATTCTCACGGTCTCCTGATCCTTACCGGTCCGACCGGTTCGGGAAAAACGACCACTCTTTATTCGCTTCTCCATTAT
GCAAAAAAACATTTTAAAAGAAACATCATCACACTTGAAGATCCTGTGGAAACGAAAAGCGATGAAATGCTTCAAGTGCA
GATCAATGAAAAAGCTGGAATTACGTATGCAAATGGGTTAAAAGCAATATTAAGACATGATCCAGATATGATTATGGTCG
GTGAAATTCGGGATCATGAGACAGCAAGGATTGCGGTCCGGGCTGGGCTTAGCGGACATTTAGTACTGACCACCATGCAT
ACGCGGGATGCAAAGGGGGCTATTTATCGATTATTAGAATTTGGTGTAACCTTTTCCGAAATTGAGCAAACTTTGATCGC
TGTTGCTGCGCAGCGTCTCGTTCATTTAATATGTCCGTTTTGCAAAGGGAATTGCCCGCCTTATTGCAAAAAATTAAAGG
AAATTAGGAGAATTGGGGTCTATGAACTTCTTTACGGCAAAAATTTAACCGCCGCTATTCGGGAAGCAAAAGGAGAGGAG
CAGAGTGAGTTAGATTATATGAAGTTAAAAGATGTAATTAAAAAAGGAATCGCTCTTGGATATTTATATCCGGACATATA
TGATCGTTGGGTGTTCGAATATGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A165XRN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

62.606

99.437

0.623
  pilB Glaesserella parasuis strain SC1401

40.058

96.338

0.386
  pilF Thermus thermophilus HB27

42.809

84.225

0.361

Multiple sequence alignment